Total serum protein determination

It will be apparent that total serum protein determinations give wholly inadequate information as to the state of the serum albumins and globulins in cirrhosis, gross disturbances in one or both fractions often escaping detection. It is important to separate the albumin fraction from the globulins, even if only crudely by the Howe method, to obtain information of value in diagnosis and prognosis. 

Know the utility of total serum protein and A/G ratio determinations and be able to solve problems involving these parameters and the results of serum protein electrophoresis determinations. 

The clinical biochemistry laboratory often determines total serum protein content and the albumin/globulin (A/G) ratio. A normal ambulatory adult will... 

The determination of total serum protein is an important clinical measurement used in diagnosing liver malfunctions. Although the Kjeldahl method is capable of high precision and accuracy, it is too slow and cumbersome to be used routinely in determining total serum protein. The Kjeldahl procedure, however, has historically been the reference method against which other methods are compared. Methods commonly used include the Biuret method and the Lowry... 

The second procedure relies on the large tryptophan content of most of the globulins compared to that of albumin (S2). The total serum proteins are precipitated, and their tryptophan content is determined by the perchloric acid reaction of Tauber (T5). Allowance is made for the small contribution of albumin to the reaction (Table 5) by standardizing the... 

All other available methods for determining serum total proteins have been discussed above in relation to albumin, and their limitations indicated. All methods suffer from differences in the individual protein s contribution of the parameter being measured. However, the tyrosine (Folin-Ciocalteu) and arginine (Sakaguchi) equivalence methods (S7) and the near-ultraviolet spectrophotometric methods are more severely affected in this respect than other methods, and are therefore unreliable for total serum protein analysis. 

D16. Drickman, A., and MoKeon, F. A., Determination of total serum protein by means of the refractive index of serum. Am. J. Clin. Pathol. 38, 392-396 (1962). 

Figure 7 NIR reflectance spectra (second derivatives) for albumin, globulins, and urea. Total serum protein may be quantified by the intensity of the serum absorption at 2064 nm, corresponding to minima (absorption maxima) in the albumin and globulin second-derivative spectra. (Adapted by permission of Elsevier Science from J.W. HaU, A. Pollard, Near-infrared Spectroscopic Determination of Serum Total Proteins, Albumin, Globulins, and Urea , Clinical Biochemistry, 483-490, Vol. 26, 1993 by the Canadian Society of Clinical Chemists.)...

Bovine gamma globulin (BGG), total protein determination BCA assay, 84 (fig.) biuret assay, 88 (fig.), 98 Coomassie plus assay, 90 Bovine serum albumin (BSA), total protein determination BCA assay, 84 (fig.) biuret assay, 88 (fig.), 98 Coomassie plus assay, 90 protein standards, 78... 

Abstract Some problems arising in the establishment of the traceability of the certified reference material (CRM) CZ 6007a (total protein, creatinine and stress indicators) are discussed. Bovine serum albumin is recommended as a calibration standard for total protein determination... 

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