Glass migration cell

Fig. 10.2 Glass migration cell for one-sided contact (courtesy FADES, Munich).

The lower surface of the haptotaxis chamber is a glass covershp modified version of a 60 X 15 mm tissue culture dish. This setup allows convenient functionahzation and washing of the cover slip, facilitates confinement, and enables imaging of the migrating cells. Commercially available glass... 

Jurkat cells have been lysed in a flow stream in a glass microchip for cell content analysis. After cell lysis, the two preloaded fluorescent dyes and their metabolites were released from the cells and separated by CE (see Figure 8.36). To prevent cell adhesion, the glass channel surface was modified by adsorbing Pluronic F-127 to the channels. In addition, to avoid blockage of adhered cell debris and to improve migration time stability, an emulsification agent, such as Pluronic P84, was added to the separation buffer [1176],... 

Low Temperature [-101 to -1°C (-150 to -l-30°F)] Cellular glass, glass fiber, polyurethane foam, and polystyrene foam are frequently used for this service range. A vapor-retarder finish with a perm rating less than 0.02 is required. In addition, it is good practice to coat all contact surfaces of the insulation with a vapor-retardant mastic to prevent moisture migration when the finish is damaged or is not properly maintained. Closed-cell insulation should not be relied... 

For borderline cases where recoveries are outside of the analytical tolerance, at say 50-70%, it is arguable whether correction of the concentrations found in the migration test for the recovery is justified. But it can be viewed to be a worst case and so in principle it is recommended. Losses may also occur due to insolubility of the substance in the food simulant or adsorption onto glass/metal surfaces and this should be investigated by rinsing the cell with a suitable solvent. 

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