Germination culture

GibberelHc acid is also used successfliUy in rice culture to promote seed germination and the growth of semidwarf varieties. The treated seed can be planted deeper than normal. In addition, the sprouting seedlings are much taller than the untreated ones they compete weU against weeds. The material is sold under the trade name Release. 

Germination Stage A lyophilized culture of M. purpurea is added to a 300 ml shake flask... 

Tank fermentation of Micromonospora inyoensis — Germination stage 1 Under aseptic conditions, add a lyophilized culture (or cells obtained from a slant culture) of M. inyoensis to a 300 ml shake flask containing 100 ml of the following sterile medium ... 

Inoculation is by conidia of A. niger or alternatively using pre-cultured mycelial pellets. Broken mycelial masses are slow to grow initially and are unsuitable here. Two to three days are required for germination during which heat input to maintain 30°C is required. During dtric add formation, cooling is necessary. 

Various assay methods have been used to detect the presence of inhibitory substances. These include some of the classical tests used by investigators of growth-promoting substances—i.e., the various Avena coleoptile assays which utilize intact, decapitated, or isolated cylinders and the split pea stem test. Effects on seed germination and seedling shoot or root growth and development have also been measured in addition to other visible expressions of inhibition. Details of many of these tests have been compiled by Mitchell et al. (99). Tests have been carried out in Petri dishes, with various solution culture techniques, and by sand and soil culture. Effects so measured may or may not be similar to those obtained under field situations— i.e., the establishment of inhibition under controlled conditions pro-... 

Flax seeds were placed for germination on moist paper for three days at 22°C and in the dark then, the plantlets were transferred under continuous white light on a liquid culture medium, as previously described [6], Suspension-cultured cells of flax were obtained from hypocotyl-derived calli as described by Schaumann et al. [4] and cultured on a medium described by Murashige and Skoog [7] containing kinetin (0.75 mg 1 ) and 2-4 D (0.2 mg 1 ). 

Isolates B9wh, CIO, 110, J20, Kll, and L16 strongly stimulated growth, germination, or both of one or more of the indicators when grown on solid medium. When grown in liquid culture, CIO, J7, and... 

The influence of nitrogen fertilizer source on the germination rate of ergots placed on the ground in rye culture has been studied. Compared with calcium ammonium nitrate, application of calcium cyanamide reduced the germination of ergots and formation of perithecia by 40-50% (Mielke, 1993). 

The whole Cladonia thalli and its derived fraction delay the spore germination and regeneration of new filaments and also inhibit their growth. Some alterations at cell level are the granular appearance and the presence of microvesicles in cytoplasm and the alterations in the shape of chloroplast, which become spherical and bulged. In cultures with whole Cladonia thalli, moss protonemal filaments are significantly shorter than... 

The temperature of incubators must be uniform throughout and should not vary more than 0.2 C. The vegetative assay organism must be sensitive to the analyte, be stable (resistant to spontaneous change), be in the logarithmic growth phase (for uniformity of response), and be easily cultured, maintained and standardized. Spores suspension have similar criteria except that the spores must be capable of germinating with reasonable synchrony. 

TPA (Knox et al., 1993). Activation of PKC by TPA prevented Fas-induced apoptosis in human leukemic T cell lines (Ruiz-Ruiz et al., 1997). Apoptosis of B cells occurring in the ileal Peyer s patch follicles in sheep could be abrogated during the first 12 hours of culture by the addition of TPA or PdBu (Motyka et al., 1993). Translocation of PKC from the cytosol rescued center B cells in germinal centers firom apoptosis (Knox and Gordon, 1994). 

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