Germ-line therapy

Davis, B.D. (1992). Germ-line therapy evolutionary and moral considerations. Hum. Gene Ther. 3,361 363. 

The chain of arguments was much more simpler in the case of germ-line therapy for disease prevention through the introduction of genes conferring resistance towards viral disease, for example, such as HIV or influenza in-... 

Uses of cloned genes Produce recombinant proteins Gene therapy (somatic) Transgenic animals (germ line) Produce cDNA probes for blots... 

Open workshop on gene therapy convened and recommended amongst other topics the need to review the safety issue relating to germ-line integration following administration of either viral or non-viral based vectors. 

Trials of gene therapy in hemophilia A and B, to determine safety and efficacy, are under way (2). Three patients with hemophilia B were treated in a phase I trial with a recombinant adenovirus-associated vector expressing human blood-coagulation factor IX (3). There was no evidence of formation of inhibitory antibodies against factor IX. In a phase I trial with a recombinant adenovirus-associated vector expressing human blood-coagulation factor IX, there was no evidence of germ-line transmission of vector sequences (3). 

See gene inherit somatic cell gene therapy germ line gene therapy. 

See genomics somatic cell gene therapy, germ line genetic mutation. See mutation. 

Specific reference is made to the application of 1990 Council Directives on the contained use of genetically modified microorganisms (8) and on the deliberate release into the environment of genetically modified organisms (9). Gene therapy trials, which could result in modifications to the germ line genetic identity of the subject, are not... 

Identifying the niches of stem cells in vivo and the requirements for stem cell viability and repopulation is essential for futme therapies in regenerative medicine, possibly by activating stem cells in situ. In Drosophila, tluee cell types act as germ line stem cell niche. 

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