Template genus

Knowledge-Based Processing with the Help of Genus Templates.120... 

FIGURE 8.8 The successfully extracted cubital cells Cj, Cj and C3 derived with the help of the superimposed genus template. 

FIGURE 8.9 ABIS the newly adjusted genus template now using the six centres of gravity of the cells B, Dj, D2, Cl, C2 and C3. The prediction of the boundaries of the radial cell is now far better than it would have been in the first superposition based only on the centres of gravity of cells B, Dj and Dj (compare with Figure 8.7). 

One advantage of this hybrid method is that the antibodies used in the immunocapture step do not need to provide the ultimate level of specificity required for the assay. For example, a genus-specific capture antibody may be used to provide the template for a species-specific PCR assay. In fact, each step of the technique can be tailored to provide the required level of discrimination. 

Human immunodeficiency virus (HIV) is a member of the retrovirus family, classified under the lentivirus genus. Retroviruses are enveloped RNA viruses, which contain a core of capsid proteins, viral RNA, and enzymes. All infectious retroviral virions contain an enzyme, reverse transcriptase, which catalyzes the formation of a complementary DNA strand from an RNA template. A double-stranded DNA copy of the viral RNA genome (proviral DNA) may then be integrated into and replicated with the host cell genome. 

Very few protein sequences in Insecta are entered into the SWISS-PROT database, and even fewer protein sequences from genus Spodoptera are found in the database (78 in Uni-ProtKB/SWISS-PROT Release 55.6, 01-July-2008). Moreover, very few GST proteins from the Lepidoptera family are available. Because none of the tryptic peptides from Spodoptera frugiperda GST matched identically to the template Manduca sexta GST), mass-spectrometry-based methods for de novo sequencing is a powerful approach for identification of unknown proteins that may be homologous to proteins from other species. 

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