Gentamicin C complex

Aminoglycoside antibiotics (257), including kanamycin A, tobramycin, and gentamicin C complex, have been acylated with 1 mole of disodium carbenicillin (258) to give monoacylated derivatives (e.g., 259) (compounds 257-259). The resulting compounds were devoid of antibacterial activity against several strains tested, however, and showed no toxicity [135]. 

Aminoglycosides are bactericidal antibiotics with a broad spectmm of antimicrobial activityIn particular, the pseudotrisaccharides of structural type 2 c (Kanamy-cin B, DKB, Amikacin, Tobramycin, Gentamicin C-complex, Sisomicin and Verda-... 

It has been noted that R factor resistance does not appear to play a major role with gentamicin (cf. Lack of phosphorylative inactivation of gentamicin C complex noted above ). 

Gentamicin Gentamicin is a complex of antibiotics isolated from a culture liquid of the actinomycete M. purpurea, which consists of a mixture of approximately equal amounts of three compounds gentamicines C, Cij, and C2 [271-278]. 

Netilmicin Sulfate, USP. Netilmicin sulfate, I -A(-cth)l-sisomicin (Netromycin), is a semisynthetic derivulivc prepared by reductive ethylation of sisomicin. an aminoglycoside antibiotic obtained from Micraiiioiiu pau iiiyoensis. Structurally, sisomicin and netilmicin rc.scniblc gentamicin C . a component of the gentamicin complex. S CHjOH... 

The solution structures of 1 1 complex between paromomycin and gentamicin Cla and the A-site oligonucleotide have been solved at high resolution using heteronuclear NMR techniques These approaches require the preparation of uniformly labeled N- and C-RNA oligonucleotides via in vitro transcription with labeled nucleoside triphosphates. The use of RNAs labeled with NMR-active nuclei allows for the application of sophisticated heteronuclear NMR methods. These methods facilitate the assigmnent of NMR resonances and the acquisition of structural restraints for detailed structure determinations. 

The components of the gentamicin complex contain C-methyl and N-methyl substituents, the source of which seems to be mainly due to methylation by L(—) methionine. The level of labelling does not completely support this argument. Reversible enzymatic reactions such as transmethylation, transamidation and deoxygenation are assumed ... 

Skin penetration and gentamicin release from the antibiotic/polymer complexes was measured in vitro by Franz diffusion cell technique [26]. The gentamicin release was studied in 500 ml of phosphate-buffered saline (PBS, pH 7.4) at 37° C in a mild shaking environment (75-100 r.p.m.). Aliquots of 3 ml were assayed for graitamicin at the time points of 0, 0.25,0.5, 0, 75,1,2,3,4, 5,6,12 and 24 h. For assessment of the quantity of gentamicin sul te released, HPLC (High-performance liquid chromatogr hy) method has been used. 

Shows broad spectrum antibacterial activity against gentamicin resistant strains. Powder. Sol. H2O, MeOH fairly sol. EtOH, Me2CO poorly sol. EtOH, hexane. Mp 102-112°. [a]o +172 (c, 0.3 in H2O). Major component of complex. 

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