Screening genotoxicity assay

Cahill PA, Knight AW, Billinton N, et al. The GreenScreen genotoxicity assay A screening validation programme. Mutagenesis. 2004 f9(2) 105-f f9. 

A pragmatic approach carry out limited toxicity studies, e.g., one 4-week rat study and one screening assay for genotoxicity with the defined mixture of (ten) priority chemicals, using exposure concentrations, e.g., 3-10 times higher than those occurring in the complex mixture... 

A positive result from a bacterial reporter assay would lower the ranking of the compound for development. The compound file should be aimotated with an alert for a confirmatory in vitro test (if the compound remains hve), in this case it might be a screening Ames at a later stage. There should also be a second alert to the lead optimization chemists. A third alert should still notify the genotoxicity safety assessment team that the compound is a priority for mechanistic investigation if the series continues, with the associated need for coordination of in vivo studies, this would be a lower priority than an Ames profiling positive. 

Elmore, E. Fitzgerald, M.P. (1990) Evaluation of the bioluminescence assays as screens for genotoxic chenticals. In Mendelsohn, M.L. Albertini, R.J., eds, Mutation and the Environment, Part D, New York, Wiley-Liss, pp. 379-387... 

---