Screen validation

Screening validation was conducted on a 1 g product basis (10 mL of product solution) with 20% w/w loading of scavenger on product at 65°C overnight. With this scale of experiment each scavenger experiment was independently filtered and the filter cake washed with toluene. The filtrate was analyzed for residual Pd by ICP/MS and for product recovery by HPLC. Results are shown in Table 5.1. For these analyses it was important to look not only at the ppm Pd in solution but also at the ppm Pd normahzed to the concentration of product, which is more reflective of... 

Reanult JY, Melcion C, Cordier A. 1989. Limb bud cell culture for in vitro teratogen screening Validation of an improved assessment method using 51 compounds. Teratogenesis Carcinog Mutagen 9 83- 96. 

Target-immobilized NMR Screening Validation and Extension to Membrane Proteins... 

High-Throughput Screens Validation for Natural Product Samples.217... 

Hodes, L. (1981a). Computer-Aided Selection of Compounds for Antitumor Screening Validation of a Statistical-Heuristic Method. JChem.Inf.Comput.Sci., 21,128-132. 

Cahill PA, Knight AW, Billinton N, et al. The GreenScreen genotoxicity assay A screening validation programme. Mutagenesis. 2004 f9(2) 105-f f9. 

Hodes, L. (1981a) Computer-aided selection of compounds for antitumor screening validation of a statistical-heuristic method. /. Chem. Inf. Comput. Sci., 21, 128-132. 

Screen validation phase tests the screening assay in a more production-like environment. For example, the lab bench results are replicated on the HTS robotic system. Critical quality control experiments are performed at this stage in the process. This involves screen rehearsal with a small number of compounds (i.e., a few thousand), thus validating the screening process. Process precision is measured by repeating the mini-screen on a different day. This procedure allows definition of all the quality control parameters. Typical values include the following ... 

Figure 16 Design of the primary screening validation experiment. Reprinted from Boussie, T. R. Diamond, G. M. Goh, C. etal. J. Am. Chem. Soc. 2003, 125 (14), 4306-4317, with permission from the American Chemical Society.

D. J. Walmsley, R. M. The Green Screen geno-toxicity assay a screening validation programme. Muta-genesis 2004, 19, 105-119. 

---