Mapping, genetic

Fig. 2. Schematic representation of the Ti-plasmid of Agrobacterium, showing the genes involved in transformation of host plants. Note that the genetic map is not to scale in reality, the T-region makes up about 10% of the total plasmid genome (from Bryant, 1988).

H21. Howard, T. D., Akots, G., and Bowden, D. W., Physical and genetic mapping of the muscle phos-phofructokinase gene (PFKM) Reassignment to human chromosome 12q. Genomics 34, 122-127(1996). 

Hanley-Hyde, J., Mushinski, J. F., Sadofsky, M., Huppi, K., Krall, M., Kozak, C. A., and Mock, B. (1992). Expression of murine cyclin B1 mRNAs and genetic mapping of related genomic sequences. Genomics 13 1018-1030. 

The genetic map is shown and the flow of events of MS2 multiplication. The infecting RNA goes to the host ribosome, where it is translated into four (or more) proteins. The four proteins that have been recognized are maturation protein (A-protein present in... 

Figure 5.17 Genetic map of phage T 7, showing gene numbers, approximate sizes, and functions of the gene products.

As seen in the genetic map, the genes after gene 1.1, transcribed by the T7 RNA polymerase, code for proteins that are involved in T7 DNA synthesis, the formation of virus coat proteins, and assembly. Three classes of T7 proteins are formed class I, made 4-8 minutes after infection, which use the cell RNA polymerase class II, made 6-15 minutes after infection, which are made from T7 RNA polymerase and are involved in DNA metabolism class III, made from 6 minutes to lysis, which are transcribed by T7 RNA polymerase and which code for phage assembly and coat protein. This sort of sequential pattern, commonly seen in many large double-stranded DNA phages, results in an efficient channeling of host resources, first toward DNA metabolism and replication, then on to formation of virus particles and release of virus by cell lysis. 

Figure 5.19 Simplified genetic map of T4. Late genes with morphogenetic functions (coat proteins and assembly), and genes with functions in DNA replication are identified. Note that although the genetic map is represented as a circle, the DNA itself is actually linear.

Figure 5.27 Bacteriophage Mu. (a) Genetic map of Mu. (Confusingly, there are two G s, the G gene and the invertible G segment. These are different G s.) (b) Integration of Mu into the host DNA, showing the generation of a five-base-pair duplication of host DNA.

Kruglyak L, Lander E. High-resolution genetic mapping of complex traits. Am J Hum Genet 1995 56 1212-1223. 

Kruglyak L. The use of a genetic map of biallelic markers in linkage studies. Nature Genet 1997 17 21-24. 

Bachmanov, A. A. et al. High-resolution genetic mapping of the sucrose octaacetate taste aversion (Soa) locus on mouse Chromosome 6. Mamm. Genome. 12 695-699, 2001. 

The availability of the sequence of the genome provides a platform for building even higher-resolution genetic maps, with the ultimate resolution being the single nucleotide. Thus recent attention has focused on building... 

These genetic maps have been the backbone for the highly successful endeavors of the molecular genetics community to identify highly penetrant... 

Geva, A., Lassere, T. B., Lichtarge, O., Pollitt, S. K., and Baranski, T. J. (2000) Genetic mapping of the human C5a receptor. Identification of transmembrane amino acids critical for receptor function. J. Biol. Chem. 275, 35393-35401. 

Reviewing the genetic map of HIV, the possible targets for antiviral activity (B2) are as follows ... 

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