Gene-specific oligonucleotides amplification

M. A. Frohmann, M. K. Dush, and G. R. Martin, Rapid production of full-length cDNAs from rare transcripts amplification using a single gene specific oligonucleotide primer, Proc. Natl. Sci. USA 1988, 85, 8998-9002. 

SEB. Therefore, serum antibody titers are of little diagnostic value. If actual bacterial involvement is suspected, and if cultures can be obtained, the detection of extremely minute quantities of potentially toxigenic strains is possible by using (1) polymerase chain reaction (PCR) amplification and (2) toxin gene-specific oligonucleotide primers. The results from both methods are rapid, allowing quantitative or qualitative measurements in less than 24 hours. 

The second experiment was carried out with the same RNA isolated from mycelia growing on presence of glucose and apple pectin. cDNAs were obtained from those RNA by reverse trancription and used as template for PCR assays. Specific oligonucleotide primers for PG gene were used in the amplification reaction. The Fig-5 shows the results of this amplification experiment. 

Marks JD, Tristem, Karpas A, Winter G, Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes, Eur. J. Immunol., 21 985-991, 1991. 

Synthesis of Genes by Polymerase Chain Reaction. Another strategy that takes advantage of specific oligonucleotide primers is the in vitro amplification of specific DNA fragments from very small amounts of an impure sample. Since the first demonstration of the technique by Mullis and Faloona (57) it has become one of the most popular procedures in molecular biology and virtually has transformed our approach to isolation of genes [for review see (58-60). 

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