Gene-specific oligonucleotides

Preininger C., Bodrossy L., Sauer U., Pichler R., Weilharter A. AR, Chip Epoxy and ARChip LTV for covalent on-chip immobilization of pmoA gene specific oligonucleotides, Anal Biochem. 2004 330 29-36. 

M. A. Frohmann, M. K. Dush, and G. R. Martin, Rapid production of full-length cDNAs from rare transcripts amplification using a single gene specific oligonucleotide primer, Proc. Natl. Sci. USA 1988, 85, 8998-9002. 

SEB. Therefore, serum antibody titers are of little diagnostic value. If actual bacterial involvement is suspected, and if cultures can be obtained, the detection of extremely minute quantities of potentially toxigenic strains is possible by using (1) polymerase chain reaction (PCR) amplification and (2) toxin gene-specific oligonucleotide primers. The results from both methods are rapid, allowing quantitative or qualitative measurements in less than 24 hours. 

Frohman MA, Dush MK, Martin GR (1988) Rapid production of fuU-length cDNAs fi-om rare transcripts ampUfication using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A 85(23) 8998-9002... 

The second experiment was carried out with the same RNA isolated from mycelia growing on presence of glucose and apple pectin. cDNAs were obtained from those RNA by reverse trancription and used as template for PCR assays. Specific oligonucleotide primers for PG gene were used in the amplification reaction. The Fig-5 shows the results of this amplification experiment. 

Mladinic M, Frederic Didelon F, Cherubini E, Bradbury A. 2000. Specific oligonucleotides often recognize more than one gene the limits of in situ hybridization applied to GABA receptors. J Neurosd Meth 98 33-42. 

Allele-specific oligonucleotide Specific alleles of a gene Genetic counseling and DNA fingerprinting... 

Figure 1-7-8. Comparison of Probes Single-Gene Versus Allele-Specific Oligonucleotide (ASO)...

Beetham, P.R. Kipp, P.B. Sawycky, X.L. Amtzen, C.J. May, G.D. (1999) A tool for functional plant genomics chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations. Proc. Natl. Acad. Sci. Usa, 96, 8774-8. 

Site-directed mutagenesis using specific oligonucleotides may be carried out either by PCR or by direct cloning using any of several proprietary kits. The gene of interest may first have to be subcloned into a suitable vector for this technique... 

Marks JD, Tristem, Karpas A, Winter G, Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes, Eur. J. Immunol., 21 985-991, 1991. 

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