Ferredoxin genes

Ta DT, Vickery LE. 1992. Cloning, sequencing, and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli. J Biol Chem 267 11120-5. 

Huber M, Garfinkel L, Gitler C, Mirelman D, Revel M, Rozenblatt S (1988) Nucleotide sequence analysis of an Entamoeba histolytica ferredoxin gene. Mol Biochem Parasitol 31 27-33... 

Multiple genes encode the enzymes involved in metronidazole reduction, and the expression of their corresponding proteins during resistance development is only known for the dominant isoforms. The potential role of other isoforms of malic enzyme, PFOR, and ferredoxin in metronidazole activation is unknown. It was found that the dominant ferredoxin (ferredoxin 1), one of seven ferredoxin genes identified in the I vaginalis genome (Carlton et al. 

Land KM, Delgadillo-CorreaMG, Tachezy J, Vanacova S, Hsieh CL, Sut ak R, Johnson PJ (2004) Targeted gene replacement of a ferredoxin gene in Trichomonas vaginalis does not lead to metronidazole resistance. Mol Microbiol 51 115-122... 

Quon DV, d Oliveira CE, Johnson PJ (1992) Reduced transcription of the ferredoxin gene in metronidazole-resistant Trichomonas vaginalis. Proc Natl Acad Sci USA 89 4402-4406... 

A fermentation process was developed for the production of epothilone B, and the titer of epothilone B was increased by a continuous feed of sodium propionate during fermentation. The inclusion of XAD-16 resin during fermentation to adsorb epothilone B and to carry out volume reduction made the recovery of product very simple (Benigni et al., 2004). A microbial hydroxylation process was developed for the conversion of epothilone B (10) to epothilone F (11) by Amycolatopsis orientalis SC 15847. A bioconversion yield of 37-47 % was obtained when the process was scaled up to 100-250 L. Recently, epothilone B hydroxylase and the ferredoxin gene have been cloned and expressed in Streptomyces rimosus from Amycolatopsis orientalis SC 15847 by our colleagues in Bristol-Myers Squibb. Mutants and variants thereof this cloned enzyme has been used in the hydroxylation of epothilone B to epothilone F to obtain even higher yields of product (Basch et al, 2004). 

Mulligan, M.E., Buikema, W.J. and Haselkom, R. (1988). Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti. J. Bacteriol. 170,4406-4410. 

Biol has now been purified and well characterized as a P-450 " and its redox partners investigated. The unique ferredoxin gene yir in the genome of B. subtilis has been overexpressed and the Fer protein, a (4Fe-4S)... 

Donadio, S. and C.R. Hutchinson (1991). Cloning and characterization of the Saccharopolyspora erythraea fdxA gene encoding ferredoxin. Gene 100, 231-235. 

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AOl. JAppl Microbiol 105 1158-1169... 

Chung BC, Guo IC, Chou SJ (1997) Transcriptional regulation of the CYPllAl and ferredoxin genes. Steroids 62 37-42... 

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