Gene-to-screen

SG Oliver. From gene to screen in yeast. Curr Opin Gen Develop 7 405-409, 1997. 

Anonymous, Functional Genomics Drug Discovery from Gene to Screen, IBC, Southborough, MA, 1997. 

Young, R. A., and Davis, R. W., 1983. Efficient isolation of genes using and-body probes. Proceedings of the National Academy of Sciences U.S.A. 80 1194-1198. Using andbodies to screen protein expression libraries to isolate the structural gene for a specific protein. 

Therefore, it is anticipated that chronic repression of muscle gene expression does not require chronic regulation by the parasite. Rather, this effect is expected to result from chronic suspension of infected cells in a non-Go/Gi gene regulatory environment. A simple hypothesis derived from known characteristics of skeletal muscle cells is that induction of cell cycle re-entry is sufficient to cause both chronic suspension in G2/M and repression of muscle gene expression. This possibility has implications for the number of regulatory points at which the parasite might influence the host cell and possible methods to screen for those products. 

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