GCSF

Filgrastim Neupogen GCSF Neutrophil 5 mcg/kg per day SQ or IV or round to 300- or 480-mcg vial size Bone pain (approximately 25%) Begin 1-3 days after chemotherapy... 

GCSF, granulocyte colony stimulating factor GMCSF, granulocyte-macrophage colony stimulating factor SQ, subcutaneous IV, intravenous AML, acute myeloid leukemia. 

Levesque JP, Hendy J, Takamatsu Y, Simmons PJ, Bendall LJ. Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide. J Clin Invest 2003 111(2) 187-196. 

Myeloid Stem Cell GCSF-R or GM-CSFR (CD114, CD115, CD116) ... 

Widera A, Kim KJ, Crandall ED, Shen WC (2003) Transcytosis of GCSF-transferrin across rat alveolar epithelial cell monolayers. Pharm Res 20(8) 1231— 1238... 

Pegfilgrasttm - conjugate of recombinant methionyl-GCSF and monomethoxypolyethylene glycol... 

This operation is illustrated by work on the human cytokine GCSF, a protein of 174 residues. The disulfide-linked loop (64-74) of this protein, thought to be involved in binding to the receptor, was replaced by various non-coded synthetic structures.[10] In this case recombinant methods were used to produce a variant of the parent protein having the appropriate cleavage sites for the production of the wanted fragments. 

Scheme 2 Construction of GCSF Analogue (l-62)-NHN=CHCO-(64-75)-NHNHCH2CO-(77-174)l10l...

The N-terminal fragment 1-62 and the C-terminal fragment 76-174 of two recombinant analogues of GCSF were isolated after enzymatic cleavage with the Lys-specific protease from Achromobacter lyticus. In one fragment, the only Lys in the protein was at position 62, and was immediately followed by Ser. In the other fragment, the only Lys, also followed by a... 

Tissue Plasminogen Activator Glucocerebroidase Therapeutic antibodies GCSF... 

Use growth factors (e.g., GCSF, GMCSF, interleukin) to stimulate hematopoiesis. 

Figure 2 Second-derivative amide I spectra of granulocyte colony stimulating factor (GCSF), a-lactalbumin, and lactate dehydrogenase (LDH) in aqueous solution (upper spectra) and dried solid (lower spectra) states. (From Ref. 11, employing data from Refs. 8 and 9.)...

The concentration of EPO (6 pmol/pl) and GCSF (10 pmol/pl) were quantified by amino acid analysis. Quantitative loading of proteins onto the sequencing supports or into the SDS-gels was done using calibrated pipettes. BLG (5 pmol/ xl) was quantitatively diluted according to Applied Biosystems recommendations. HSA (1.11 pmol/gl) was used as received from Hewlett Packard. 

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