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Multisite Gateway

Panel B). For example, by reversing the standard orientation of the attPl site (to an attPlr site), a BP recombination with an attBl site (in reverse orientation, attBlr) results in the formation of an attRl site instead of an attLl site. This concept is key to the elegance of the Multisite Gateway system. [Pg.617]

Fig. 2.8 Three-fragment Multisite Gateway cloning. Creation of entry vectors and three-fragment Multisite assembly reaction. Fig. 2.8 Three-fragment Multisite Gateway cloning. Creation of entry vectors and three-fragment Multisite assembly reaction.
The full power of this system is realized in the Multisite Gateway system, where the same mechanism which is used to transfer single DNA elements from vector to vector is employed to assemble multiple fragments. The future of Gateway cloning will include HTP, controllable assembly of multi-element plasmids, multi-domain proteins, and entire enzymatic pathways that could be expressed in a desired cellu-... [Pg.619]

Gateway entry clones 611 Gateway, multisite 616 Gateway reactions 610... [Pg.1857]

See other pages where Multisite Gateway is mentioned: [Pg.613]    [Pg.616]    [Pg.617]    [Pg.617]    [Pg.613]    [Pg.616]    [Pg.617]    [Pg.617]    [Pg.617]    [Pg.1857]   


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