Gas/liquid chromatography/mass spectrometry GLC/MS

J. N. Damico and R. P. Barron. Application of Field Ionization to Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) Studies. Anal. Chem., 43(1971) 17-21. 

ECD = electron capture detector GC = gas chromatography GLC = gas liquid chromatography MS = mass spectrometry... 

The picolinyl esters (32) permit the location of double bonds, since these give distinctive fragmentations that are characteristic of the double-bond positions. They are easily prepared and are not too polar for separation by gas-liquid chromatography (GLC). It has been confirmed that the picolinyl esters are the most useful, since they permit unequivocal identifications even with polyunsatured components. It was also demonstrated that derivatives of this type, prepared from natural mixtures, give satisfactory resolutions when subjected to GLC on capillary columns of fused silica coated with a nonpolar methylsilicone phase, for identification by mass spectrometry (MS). 

Abbreviations GLC, gas-liquid chromatography MS, mass spectrometry HPLC, high performance liquid chromatography IR, infra-red DEAE, diethylaminoethyl NMR, nuclear magnetic resonance TLC, thin layer chromatography. 

Note TLC, thin-layer chromatography HPLC, high-performance liquid chromatography GLC or GC, gas-liquid chromatography AA, atomic adsorption NPD, nitrogen phosphorus detector FPD, flame photometric detector GC/MS, gas chromatography/mass spectrometry. 

There are two types of GC gas-solid (adsorption) chromatography and gas-liquid (partition) chromatography. The more important of the two is gas-liquid chromatography (GLC), used in the form of a capillary column. In this chapter, we describe the principles of operation of gas chromatography, the types of GC columns, and GC detectors. The principles of mass spectrometry (MS) are described, along with coupling of the gas chromatograph with a mass spectrometer (GC-MS). 

Analytical techniques used to quantify either total or individual bile acids in biological fluids include gas-hquid chromatography (GLC), high-performance liquid chromatography (HPLC), enzymatic assay, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and tandem mass spectrometry (MS/MS). 

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