Galactocerebrosides synthesis

Bosio, A. Binczek, E. Stolfel, W. Functional breakdown of the hpid bilayer of the myelin membrane in central and peripheral nervous system by disrupted galactocerebroside synthesis. Proc. Natl Acad. Sci. U.S.A. 19%, 93 13280-13285. 

The assays for the enzyme synthesis of sulfogalactosyl-glycerolipid, sulfatides and galactocerebrosides were carried out as previously described respectively by Subba Rao, et al. (28) Sarlieve, et al. 05, 29), and Neskovic, et al. (30). The assay for 2, 3 cyclic nucleotide phosphohydrolase was performed according to the method of Prohaska, et al. (31). EL coli. alkaline phosphatase type III-S, 2, 3 -cAMP, and sodium deoxycholate were obtained from Sigma (St. Louis, Mo.). Protein was determined by the method of Lowry, et al. (32) with crystalline bovine serum albumin as the standard. 

The vitamin also activates serine palmitoyltransferase, the first enzyme of phosphosphingolipid synthesis, and in bacteria it can, together with inorganic phosphate, replace part of the ATP requirement of galactocerebroside sulfo-transferase (Tsaioun, 1999). In animals, lipid sulfatides are decreased in vitamin K deficiency and increased with higher intakes (Sundaram et al., 1996). 

Basu S, Schultz A, Basu M, Roseman S (1971) Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain. J Biol Chem 246 4272-4279... 

GalT-1 or CGalT(UDP-Gal Ceramide p 1,1 galactosyl transferase Table 2 [33, 51-54]) 2-hydroxy-fatty acid catalyzes the synthesis of galactocerebroside (gal-actosylceramide), which is the major constituent of myelin membranes. The localization of GalT-1 and its role in membrane sorting is recently being explored [86]. 

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