Production fusion

Protein kinase B, or Akt, was discovered as the product of an oncogene of the acutely transforming retrovirus AKT8, causing T-cell lymphomas in mice. It encodes a fusion product of a cellular serine/threonine protein kinase and the viral structural protein Gag. This kinase is similar to both protein kinase Ce (PKCe 73% identity to the catalytic domain) and protein kinase A (PKA 68%). It differs from other protein kinases in that it contains a pleckstrin homology (PH) domain, which allows it to bind to polyphosphoinositide head groups (and also to G-protein fly subunits). To date, three subtypes have been identified a, (3, and y, all of which show a broad tissue distribution. It... 

Since a monoclonal antibody is a fusion product of a malignant mouse cell and an antibody-producing cell, there is some concern about the safety of the production process itself (Petricciani, 1983). Methods for the production of monoclonal antibodies raise two general safety issues (1) the theoretical risk of transferring in the product factors associated with malignancy (e.g., oncogene factors) and (2) the use of animals for antibody production that are known to harbor a number of microbial agents some of which can produce diseases in humans. 

While the popularity of antibody-antigen microarrays remains on the increase, other approaches are also useful in proteomic studies. Phylos technology (Weng et al., 2002) makes use of mRNA-protein fusion products that... 

L- and DL-dihydro-orotic acid supported growth of Lactobacillus bulgaricus 09. The D-isomer alone is not only without activity, but is reported to reversibly inhibit the growth-promoting property exerted by ureidosuccinic or orotic acid [74, 91]. The dihydro-orotic acid prepared by fusion of maleic acid and urea [130] is inactive in bothi. bulgaricus (B [128] and an enzyme system [72, 131]. A comparison of the urea fusion product and that prepared by catalytic hydrogenation of orotic acid revealed that the former is actually fumarylurea [132]. 

The first step towards an answer is once again the fragility and instability of helium s fusion products. Why then is helium so stable whilst its offspring are so fragile Why are nuclei with masses 5 and 8 times the proton mass so unsure of themselves that they have disappeared from the map of the world The explanation for this can be found in the microarchitecture of the atomic nucleus, a subject that is hardly conducive to literature. 

Elemental oxygen also is present in the sun in less than 1% mass composition, as a fusion product of carbon-12, and hehum-4. No planet or its moon in the solar system, other than the earth is known to contain molecular oxygen in its atmosphere, although CO2 is a major component of many planetary... 

Cd azides, Ag acetylide and nitrogen iodide) by electrons, neutrons, fusion products and X-rays. All these substances were exploded by an intense electron stream but it was shown that this was due to a thermal effect. Fission products exploded nitrogen iodide but in the other substances some changes within the crystals took place but no explosions. The experiments showed that, in general, the activation of a small group of adjacent molecules was not enough to cause explosion... 

Dihydroxyindole and 5,6-dihydroxyindole-2-carboxylic acid were shown to form after the red pigment stage that occurred during the conversion of DOPA into a melanin, by Raper, who isolated these compounds as their dimethyl ethers.72 The presence of 5,6-dihydroxyindoles in solutions of DOPA, dopamine, and noradrenaline which are undergoing oxidation has subsequently been confirmed by paper and thin-layer chromatography.118,120,222 5,6-Dihydroxyin-dole and 5,6-dihydroxyindole-2-carboxylic acid have recently been isolated from the alkali fusion products of sepiomelanin, indicating... 

Sulphur and Iodine.—Various methods have been described for the preparation of compounds of iodine and sulphur,3 but to-day the products are regarded merely as mixtures of the elements.1 In solution in carbon disulphide, iodine and sulphur exist side by side permanently uncombined. The freezing-point curves for mixtures of the two elements,5 as also the vapour pressure curve of the fusion products,6 likewise give no indication whatever of chemical combination, although sulphur forms a solid solution in iodine. A further proof of the absence of combination is the fact that when dissolved in iodine, sulphur has a normal molecular weight, determined eryoseopically, only a little below that required for S8.7 The present condition of our knowledge, therefore, may be summed up in the statement that no definite compound of sulphur and iodine has yet been obtained.8... 

Once transfectomas have been generated, they must be screened for the production of the desired fusion. The binding site provided by the expression of the VNP heavy chain in J558L cells and association of the heavy chain with the resident light chain means that the protein fusion can be captured on a solid phase by NP or NIP, and detected using commercially available antibody to mouse X chain and an appropriate enzyme conjugate, in a simple ELISA screening procedure. Similarly, purification can be achieved by affinity adsorption of the fusion onto an NP matrix. An immunoblot method is described here for characterization of selected transfectomas, which allows the mol-wt of the fusion product to be estimated. 

Screening by ELISA for Ceil Lines Expressing and Secreting the Fusion Product... 

Xgt-11 Infection of E. coli Strain C600 for Western Blot Analysis of (3-Galactosidase-cDNA Gene Fusion Products... 

When these heavy recoil nuclei are the result of a complete fusion of the projectile and target nuclei, they are usually called evaporation residues because they result from a deexcitation of the primary complete fusion product by particle evaporation (emission). In intermediate energy and relativistic nuclear collisions, the momentum transfer to the target nucleus is much less, and the energy of the recoiling nucleus is 5-100 keV/nucleon. Such recoils are usually called heavy residues ... 

The main appeal of nuclear fusion as a power source is that the hydrogen isotopes used as fuel are cheap and plentiful and that the fusion products are nonra-dioactive and nonpolluting. The technical problems that must be solved before achieving a practical and controllable fusion method are staggering, however. Not the least of the problems is that a temperature of approximately 40 million kelvins is needed to initiate the fusion process. 

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