Furazolidone methods

Apart from differences between muscle tissues from various parts of an animal, there are qualitative and quantitative differences in composition between animal species. Therefore, analytical methods will always have lo be tested on material from each individual species, since differences in fat composition, in the presence of species-specific proteins, and in colored components such as in the case of myoglobin in poultry and beef may influence both the extraction and the separation of the analytes. As an example, a recovery higher than 70% was obtained for furazolidone after spiking chicken and veal calf muscle tissue but only 10% after spiking pork tissue (16). In this study, the recovery from pork meat could markedly be improved by addition to the aqueous exfraction solvent of about 25% acetonitrile, an observation indicating binding of furazolidone to pork-specific proteins. 

A multiresidue preparation technique—MSPD—has also been applied to the analysis of CAP residues in meat samples. Two fractions were collected by elution with methylene chloride and ethyl acetate. No additional purification was necessary. Diode assay detection and fluorescence detectors were recommended for the multiresidue analysis of sulfonamides, benzimidazoles, nicarbazin, furazolidone, and CAP. The percentage recoveries and linearity of the method were evaluated. The method was linear from 50 to 250 /tg/kg of CAP. Not only do the authors recommend the MSPD multiresidue procedure for HPLC analysis, but it could be associated with several detection modes, such as immuno- or receptor assays. The MSPD technique represents a new approach in the field of biological-matrix extraction and provides a great possibility for the analysis of a wide range of compounds (20). 

V Hormazdbal, M Yndestad. Simple and rapid method of analysis for furazolidone in meat tissues by high-performance liquid chromatography. J Liq Chromatogr 18 1871-1877, 1995. 

Furazolidone, furaltadone Alkaline hydrolysis 1-20 Use of method of tangents... 

UV detection and diode-array detection (DAD) have been widely used, coupled to HPLC, for the analysis of the parent compounds of nitrofurans. The detection wavelengths of nitrofiirans have been set at 368 nm," 365 nm, " 360 nm," 270 nm, or at 254 nm. " An HPLC method, with electrochemical detection, has also been described for the monitoring of furazolidone and furaltadone hydrochloride in chickoi tissues and eggs. " ... 

Alawi, M.A. Determination of furazolidone and furaltadone in Jordanian local eggs using a modified HPLC method. Fresenius Environ. BuU. 1999, 8, 86-93. 

Petz, M. Method for determination of residues of furazolidone and four other nitrofurans in eggs, milk, and meat by HPLC. Deutsche Lebensmitt. Rund. 1982, 78, 396-401. 

Argekar, A.P. Shah, S.J. A fast and accurate HPLC method for the simultaneous determination of metronidazole (MET) and furazolidone (FURA) in tablets. Indian Drugs 1998,35, 71-74. 

Gips, M. Bridzy, M. Barel, S. Soback, S. A high performance hquid chromatography method for detection of chloramphenicol, nitrofurazone, and furazolidone residues... 

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