Genomics functional cloning

Skjot, M., Kauppinen, S., Kofod, L. V., Fuglsang, C., Pauly, M., et al., Functional cloning of an endo-arabi-nanase from Aspergillus aculeatus and its heterologous expression in A-oryzae and tobacco. Molecular Genetics Genomics 2001, 265 (5), 913-921. 

De Miranda J, Santoro A, Engelender S, and Wolosker H (2000) Human serine racemase molecular cloning, genomic organization and functional analysis. Gene 256,183-8. 

Carninci P, Shibata Y, Hayatsu N, et al. (2001). Balanced-size and long-size cloning of full-length, cap-trapped cDNAs into vectors of the novel lambda-FLC family allows enhanced gene discovery rate and functional analysis. Genomics. 77 79-90. 

Genomic RNA. The viral genome is a positive-stranded RNA of approximately 11,700 nucleotides and has the structural features of messenger RNA (ie, mRNA, a 5 methylated cap [m7GpppA] and a poly-A tract at the 3 end).77 As a complete and functional mRNA, genomic RNA purified from virions is fully infectious when artificially introduced (ie, transfected) into susceptible cells. Similarly, RNA transcribed from a full-length complementary DNA (cDNA) clone of an alphavirus is also infectious,... 

Farm other animal genomes Seq variation in chicken genome Mouse cDNA clone functional annotation Genomic align, annotation, exptl results Homologous genes in complete euk genomes... 

Simonin F., Gaveriaux-Ruff C., Befort K. et al. k-Opioid receptor in humans cDNA and genomic cloning, chromosomal assignment, functional expression, pharmacology, and expression pattern in the central nervous system. Proc. Natl. Acad. Sci. U.S.A. 92 7006, 1995. 

The peppermint oil gland secretory cell cDNA library has proven to provide a highly enriched source of candidate genes involved in essential oil biosynthesis. A functional genomics approach has successfully been employed to clone genes involved in the mevalonate-independent pathway of isoprenoid biosynthesis and in the peppermint-specific steps producing (-)-menthol and (-)-menthone. The optimization of LC-MS technology to profile phosphoiylated carbohydrates and... 

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