Functional analysis thin layer chromatography

Sarbu, C., and Cobzac, S. (1997). Validation of the linear calibration function in thin layer chromatography by analysis of variance. Rev. Chim. (Bucharest) 48 239-245. 

Thin-Layer Chromatography TLC) The function of TLC in organic synthesis is primarily one of allowing the experimenter to follow the progress of the reaction without actually interrupting the reaction. Since successful TLC can be carried out on a minute scale, only a very small fraction of the reaction mixture need be withdrawn and subjected to analysis. The following example of the TLC analysis of the chromic acid oxidation of borneol, described by Davis (3), is a useful model. 

Another qualitative technique used to characterize functionalized polymers is thin layer chromatography (TLC). ° Just as small molecules can be separated based on their affinity for a support and solvent, functionalized polymers can be separated from nonfunctionalized products. Polymers are characterized in the same manner as small molecules to obtain the relative amounts of the components in a mixture. In fact, it has been shown that TLC analysis can be used to detect as little as 1-2% of unfunctionalized polymer in a mixture. Provided that the components of the mixture can be separated as determined by TLC, column chromatography can be used to separate the components of the mixture in order to determine the weight percent of each component. In this manner, TLC combined with column chromatography can lead to quantitative... 

A quantitative TLC technique has also been used to determine the functionality of polymers. This technique, thin layer chromatography-flame ionization detection (TLC-FID), is a specialized technique that requires more advanced equipment than standard TLC. In order to perform TLC-FID analysis of a polymer mixture, specially designed thin quartz rods coated with Si02 are used. Chromatographic separation of the mixture is performed on the rod analogous to traditional TLC. After separation, the rod is scanned with sufficient heat to ionize each component of the separated spots. The ionized spots are then quantitatively detected by a flame ionization detector. It is reported that this method has an error limit of about 2%. ... 

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