Neonatal foreskin

B.M. Sutherland used in vitro experiments with human neonatal foreskin fibroblasts in buffer solution to show that DNA-pyrimidine dimer formation under a sunlamp was sensitized by p-aminobenzoic acid. Cells illuminated in the presence of the acid had a 10-fold higher frequency of transformation to anchorage-independant growth than cells irradiated in buffer alone [41]. 

Among the commercially available 3D skin models suitable for conducting such test and providing sufficient cell proliferation is the EpiDerm (MatTeK Corporation, Ashland, MA, USA). The model is constructed from primary neonatal epidermal foreskin-derived keratinocytes. It forms a multilayer differentiated tissue, which contains the dividing basal cell layer along with spinous, granular, and cornified layers resembling the normal human epidermis. DNA repair mechanism, cell cycle control, and checkpoint mechanisms and metabolism are much more similar to the normal conditions in human than those of transformed cell lines. 

Although microscopic descriptions of World War I vesicant agent-induced lesions exist, more recently Papirmeister et al. (1985) have described a model (full-thickness human neonatal foreskin grafted to congenitally athymic nude mice) which appears to reproduce very accurately the findings previously described. This model has also allowed lesions to be studied at the ul-trastructural level in an attempt to reveal additional information about the exact histogenesis of vesicant-induced cutaneous injuries. The observations made from this model can be summarised as follows. 

Pashn, D. A., Reykjalin, E., Tsadik, E., Schour, L., Lucas, A. (2015). A MoUuscum con-tagiosum fusion protein inhibits CCLl-induced chemotaxis of cells expressing CCR8 and penetrates human neonatal foreskins Clinical appHcations proposed. Archives of Dermatological Research, 307, 275-280. 

Bikle D, Nemanic M, Whitney J, Elias P (1986) Neonatal human foreskin keratinocytes produce... 

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