Flux balance analysis metabolic model

Fig. 2 Simplified workflow for the reconstruction of genome-scale metabolic models and their use in simulating cellular function through flux balance analysis...

Metabolic networks can be quantitatively and qualitatively studied without enzyme kinetic parameters by using a constraints-based approach. Metabolic networks must obey the fundamental physicochemical laws, such as mass, energy, redox balances, diffusion, and thermodynamics. Therefore, when kinetic constants are unavailable, cellular function can still be mathematically constrained based on the mass and energy balance. Flux balance analysis (FBA) is a mathematical modeling framework that can be used to study the steady-state metabolic capabilities of cell-based physicochemical constraints. ... 

Whereas detailed dynamic models can precisely answer questions on cellular behavior, the widespread application of such approaches has been hampered by the lack of kinetic information. In the absence of kinetic information, a method known as flux balance analysis (FBA) has been developed to analyze the metabolic capabilities of a cellular system based on mass balance constraints [Varma and Palsson 1994 Edwards et al. 1999 Edwards and Palsson 2000],... 

Due to lack of kinetic parameters, structural metabolic network modeling has been widely applied for analyzing cellular metabolism under steady-state. Depending on what assumptions are made and whether experimental data are required, different techniques have been developed to analyze the invariant of metabohc networks such as metabolic flux analysis (MFA), flux balance analysis (FBA), and metabolic pathway analysis (MPA) including elementary mode and extreme pathway analyses (Lewis et al. 2012 Stephanopoulos et al. 1998 Trinh et al. 2(X)9). 

A more popular form of stoichiometric analysis is the analysis of flux distributions that are consistent with system steady state (Note that in the terminology of metabolic modelling, the rate of a reaction at system steady state is referred to as a flux.) This type of analysis can be done directly on the N matrix because of its central role in the description of the mass balances of all the variable intermediates in a network. 

Metabolic flux analysis Cellular metabolites and metabolic fluxes can be combined into a series of balance equations, not unlike a series of (bio)chemical reactions in a kinetic model. Metabolic flux analysis is the description of the components and their connections in a metabolic network. 

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