Fluorometric assay estimation

The enzyme activity is used as an index to estimate the enzyme s potential to produce the desired product. Different physicochemical techniques are available to measure the activity, either by substrate consumption or product formation (Smeltzer et al, 1992), For a good estimate of the activity, the range of substrate concentrations should be carefully selected and accurately measured. Continuous assays, which continuously monitor changes in the reaction solution s physical properties, such as light absorbance (colorimetric), fluorescence (fluorometric), or heat release/ absorbance (calorimetric), are among the possible techniques. Discontinuous assays, where samples from a reaction solution are collected at intervals, and the amount of substrate or product concentrations are measured, are also frequently used. 

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