Reagents fluorogenic

Ito, K., and Maruyama, J. (1983) Studies on stable diazoalkanes as potential fluorogenic reagents. I. 7-substituted 4-diazomethylcoumarins. Chem. Pharm. Bull. 31, 3014. 

The 3-(2-furoyl)quinoline-2-carbaldehyde has been used as a fluorogenic reagent for the analysis of primary amines by liquid chromatography with laser-induced fluorescence detection [10,11]. 

There are various alternatives to obtain fluorescence with a pesticide. For instance, fluorescence may be derived from the pesticide by treatment with heat, acid, base, inorganic salts or a combination of these. Another approach is to prepare a derivative Cfluorogenic labelling) in solution the derivative is then extracted and applied directly on a chromatogram for separation. A fluorogenic reagent that will become fluorescent on contact with the pesticide may also be used. These alternatives are summarized in Figure 5. 

Flavones (fluorescence enhancement). Flavones have been used to detect pesticides on cellulose thin-layer chromatograms (2 1). Fluorogenic reagents such as Fisetin (III) are relatively non-fluorescent on a non-polar background like cellulose. The presence of... 

Royo S, Martmez-Manez R, Sancenon F, Costero AM, Parra M, Gil S. Chromogenic and fluorogenic reagents for chemical warfare nerve agents detection. Chem Commun 2007 4839-47. 

Miller I, Crawford J, Gianazza E. Protein stains for proteornic applications which, when, why Proteomics 2006 6 5385-5408. Mohr GJ. New chromogenic and fluorogenic reagents and sensors for neutral and ionic analytes based on covalent bond formation—a review of recent developments. Analyt. Bioanalyt. Chem. 2006 386 1201-1214. 

Propranolol, metoprolol and atenolol derivati/ed with a fluorogenic reagent, 4- N-chloroformylmethyl-A -methyl)amino-7- V,A -dimethylaminosulphonyl-2.1.3-ben/oxadia/olc... 

Most amino acids react with ninhydrin at ambient temperatures to form a blue color that becomes purple on heating. However, proline and hydroxyproline yield yellow compounds that are measured at a different wavelength. Other postcolumn derivatizations use fluorogenic reagents, such as o-phthaldialdehyde or fluorescamine. Precolumn derivatization techniques using o-phthaldialdehyde, dansyl, phenyl isothiocyanate, or 9-fluorenylmethyl chloroformate derivatives have been used with reversed-phase HPLC. Electrochemical detection has also been coupled with derivatization methods to enhance analytical sensitivity. 

The substrate for this assay consists of hyaluronan with the reducing terminals labeled with the fluorogenic reagent 2-aminopyridine [144], It should be noted that the reaction products by hyaluronidase also contain oligosaccharides that do not contain the fluorogenic end group. As these products are not detectable by this method, not all scissions occurring in the substrate are detected. 

Chen, P. and M.V. Novotny. 1997. 2-Methyl-3-oxo-4-phenyl-2,3-dihydrofuran-2-yl acetate a fluorogenic reagent for detection and analysis of primary amines. Anal. Chem. 69 2806-2811. 

PHWE-LC systems have been utilized, for example, to determine N-methylcarbamates in food. PHWE was done at 75 °C and a combination of static and dynamic extraction was used. In this system, the effluent from the chromatograph was coupled with a flow injection manifold where the N-methylcarbamates were hydrolyzed and then derivatizatized by reaction with a fluorogenic reagent (o-phthalaldehyde). The derivatized analytes were driven to a fluorimeter equipped with a flow-cell allowing monitoring (31). 

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