Fluorogenic detection reagents

The identification of unknown pesticide zones is initially based on the comparison of the migration of sample zones relative to standards developed on the same layer and colors obtained with selective chromogenic and fluorogenic detection reagents. Many densitometers can record in situ UV and visible absorption and fluorescence excitation spectra to confirm compound identification by the comparison of unknown spectra with stored standard spectra obtained under identical conditions or spectra of standards measured on the same plate. Additional confirmation methods include off-line and on-line combination of TLC with infrared, Raman,... 

Liquid chromogenic and fluorogenic detection reagents such as those in Table 1 can be applied by spraying or... 

Davani, B., and Olsson, N. U. (1995). Detection of natural galactolipids by thin layer chromatography with 8-anifino-l-naphthalenesulfonate (ANS) as fluorogenic visualization reagent. J. Planar Chromatogr—Mod. TLC. 8 33-35. 

Enzymes can be linked to immunoassay reagents to amplify detection by the use of fluorogenic substrates. Enzyme-linked fluoroimmunoassays (ELFIAs) are very similar to photometric EIAs in format and workflow. EIAs are widely used, and many commercial ELFIA assays and systems are available/15 The most commonly used enzymes in ELFIAs are horseradish peroxidase, alkaline phosphatase, and fi-D-... 

ELISA of sheep IgM (antigen) was conducted in a PDMS chip. The capture antibody (rabbit anti-sheep IgM-HRP) was added. Then the fluorogenic substrate (HPPA) was added for fluorescent detection. The conventional blocking reagents (0.5% w/v BSA, 0.5% w/v casein, 0.5% v/v Tween-20), which normally worked for the polystyrene ELISA plate, did not work with the hydrophobic PDMS surface. To solve this problem, 4% PEG and 7% normal rabbit serum were included in the above solution for effective blocking [173]. 

The 3-(2-furoyl)quinoline-2-carbaldehyde has been used as a fluorogenic reagent for the analysis of primary amines by liquid chromatography with laser-induced fluorescence detection [10,11]. 

Flavones (fluorescence enhancement). Flavones have been used to detect pesticides on cellulose thin-layer chromatograms (2 1). Fluorogenic reagents such as Fisetin (III) are relatively non-fluorescent on a non-polar background like cellulose. The presence of... 

Royo S, Martmez-Manez R, Sancenon F, Costero AM, Parra M, Gil S. Chromogenic and fluorogenic reagents for chemical warfare nerve agents detection. Chem Commun 2007 4839-47. 

---