Fluorescently-labelled compound

In the second series, the fluorophore NBD was linked to the carboxy terminus via a piperazine spacer as in 188 or via 6-(ethylamino)-iV-butyl-l,8-naphthalimides and yielded the fluorescently labeled compound 189 . ... 

Nucleophilic ring opening of salicyl alcohol acetals was effected by borane-trimethylamine upon Lewis acid activation with AICI3. This reaction was particularly useful for the construction of fluorescently labeled compounds (Equation 29) <2006JOC3444>. 

An indication of the potential sensitivity of fluorometry is that single-molecule detection has been based almost exclusively on the use of fluorescent labeled compounds. In addition, fluorometric determinations can combine several parameters simultaneously ( multidimensional techniques), such as excitation and emission wavelengths, fluorescence lifetime, and polarization, providing additional specificity and versatility to the analytical measurements. 

Figure 7.13 The response of a biosensor system utilizing a competitive analog analyte S, e.g. a fluorescently labeled compound. 

FIGURE 21.15 Structure of G i. A fluorescently labeled compound has been prepared where the ceramide group (box) is replaced with the TMR-label (bottom box). 

The fluorescent labelling compound 2-amino-6-amidobiotinylpyridine can be used to follow oligosaccharides during purification and complex formation improved methodology has been descsribed. ... 

Fujita, S. Reddi, P. J. Tom, T. Fluorescent labeling compounds for amino acid-containing compounds. Jpn. Kokai Tokkyo Koho JP 10330299,1988. 

Elimination from the vitreous occurs by one of two pathways. This can be visualized by injecting fluorescent compounds and examining the concentration distribution in frozen sections obtained after a steady state has been established [230]. If the major route of elimination is by means of the re-tina/choroid, at steady state the lowest concentration would be in the vicinity of the retina. The contours observed in frozen sections of the rabbit eye obtained after intravitreal injection of fluorescein exhibit this pattern, with the highest concentration immediately behind the lens (Fig. 16A). Compounds not chiefly eliminated through the retina exit the vitreous by passive diffusion and enter the posterior aqueous, where they are eliminated by the natural production and outflow of aqueous humor. In such a situation, the contours would be perpendicular to the retina, with the highest concentration towards the rear of the vitreous cavity. This appears to be the case for fluorescently labeled dextran polymer, whose contours decrease in concentration toward the hyaloid membrane (Fig. 16B). 

The commercially available dicyanomethylene squaraine dye Seta-670-mono-NHS showed extremely low blinking effects and good photostability when used in single-molecule studies of multiple-fluorophore labeled antibodies [113]. Seta-670-mono-NHS and Seta-635-NH-mono-NHS were covalently labeled to antibodies and used in a surface-enhanced immunoassay [114]. From the fluorescence intensity and lifetime changes determined for a surface that had been coated with silver nanoparticles, both labeled compounds exhibited a 15- to 20-fold... 

Hydroxy or hydroxymethyl groups attached to quinolizine substrates normally show the expected reactivity. For instance, compound 109 was linked to histamine receptor antagonists by esterification with succinic anhydride followed by amidification with the antihistaminic drug to give the fluorescently labeled stmcture 110 for in vivo studies of receptor binding (Scheme 13) <2003BML1717>. 

In order to work out in more detail Harada and coworkers took a step further by preparing fluorescence-labeled bis(l-naphthylacetyl)-PEG (PEG-1N2) and bis(2-naphthylacetyl)-PEG (PEG-2N2). The complexes of these compounds with y-CD were isolated by standard methods and subjected to NMR measurement. The results also conformed to the conclusion that four ethylene glycol units are included in one y-CD. 

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