Fluorescent, labeling reagent

Khalfan, H., Abuknesha, R., Rand-Weaver, M., Price, R.G., and Robinson, D. (1986) Aminomethyl cou-marin acetic acid A new fluorescent labeling reagent for proteins. Histochem. /. 18, 497-499. 

A fluorescence labeling reagent (Cydye DICE fluors, Cy5 Minimal, fi om GE Healthcare UK Ltd.) is dissolved into N,N-dimethylformamide (DMF) and adjusted to 400 pmol/pL of concentration. 

Particle Concentration Fluorescence Immunoassay. The PCFIA is a solid-phase immunoassay in which proteins are attached to polystyrene particles by adsorption or covalent coupling for the solid phase and fluorescent-labeled reagents are utilized for product detection.22 The general principles of the assay are similar to those of the enzyme-linked immunosorbent assay (ELISA), which has been reviewed extensively elsewhere.23 PCFIAs are performed in specially designed 96-well format Fluoricon assay plates utilizing an automated Screen Machine (Idexx Corporation, Research Product Division, Portland, ME). 

Lippolis, V., Pascale, M., Maragos, C.M., Visconti, A. (2008). Improvement of detection sensitivity of T-2 and HT-2 toxins using different fluorescent labeling reagents by high-performance liquid chromatography. Talanta 74 1476-83. 

Gatti, R. Cavrini, V. Roveri, R 2-Bromoacetyl-6-methoxynaphthalene A useful fluorescent labelling reagent for HPLC analysis of carboxylic acids. Chromatographia, 1992, 33, 13—18... 

Fig. 1- Common reactions of fluorescent labeling reagents. T represents the target in the labeling reaction and can be a protein, drug, or modified nucleic acid. F represents the fluorescent label.

T Yoshida, A Uetake, C Nakai. Liquid chromatographic determination of biotin by using 1-pyrenyldiazomethane as a pre-column fluorescent labelling reagent. J Chromatogr 456 421-426, 1988. 

Tsuruta, Y. Date, Y. Kohashi, K. (2-Phthalimidyl)benzoylazides as fluorescence labeling reagents for alcohols in high-performance liquid chromatography, A a/.Sci., 1991, 7, 411-414. 

Akasaka, K. Suzuki, T. Ohrui, H. Meguro, H. Shindo, Y. Takahashi, H. 9-Bromomethylacridine a novel fluorescent labeling reagent of carboxylic group for HPLC, Anal.Lett., 1987, 20, 1581-1594. [caprylic acid capric acid lauric acid linolenic acid arachidonic acid palmitoleic acid myristic acid linoleic acid oleic add palmitic acid stearic add]... 

Nimura, N. Kinoshita, T. Yoshida, T. Uetake, A. Nakai, C. 1-Pyrenyldiazomethane as a fluorescent labeling reagent for liquid chromatographic determination of carboxylic acids, Anal.Chem., 1988, 60, 2067-2070. [derivatization gradient isocratic fluorescence detection LOD 20-30 fmol lactic acid formic acid propionic acid dinoprostone dinoprost alprostadil linolenic acid palmitoleic acid myristic acid linoleic acid palmitic acid oleic acid stearic acid arachidic acid]... 

Yoshida, T., Uetake, A., Nakai, C., Nimura, M., and Kinoshita, T., Liquid chromatographic determination of biotin using Ipyrenyldiazomethane as a precolumn fluorescent labeling reagent, J. Chromatogr., 456, 421-426, 1988. 

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