Fluorescence resonance energy transfer based

Hoppe, A., Christensen, K. and Swanson, J. A. (2002). Fluorescence resonance energy transfer-based stoichiometry in living cells. Biophys. J. 83, 3652-64. 

Goldman ER, Medintz IL, Whitley JL et al (2005) A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor. J Am Chem Soc 127 6744-6751... 

Goldman, E. R., I. L. Medintz, J. L. Whitley, A. Hayhurst, A. R. Clapp, H. T. Uyeda, J. R. Deschamps, M. E. Lassman, and H. Mattoussi. A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor. J. Am. Chem. Soc. 127, 6744-6751 (2005b). 

Koo, K., and Jaykus, L.-A. (2003). Detection of Listeria monocytogenes from a model food by fluorescence resonance energy transfer-based PCR with an symmetric fluorogenic probe set. Appl. Environ. Microbiol. 69,1082-1088. 

Azpiazu, I., and Gautam, N. (2004). A fluorescence resonance energy transfer-based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell./. Biol. Chem. 279, 27709-27718. 

Buranda, T., Lopez, G. P., Simons, P., Pastuszyn, A., and Sklar, L. A. (2001). Detection of epitope-tagged proteins in flow cytometry Fluorescence resonance energy transfer-based assays on beads with femtomole resolution. Anal. Biochem. 298, 151-162. 

Ballerstadt R, Gowda A, McNichols R. Fluorescence resonance energy transfer-based near-infrared fluorescence sensor for glucose monitoring. Diabetes Technology Therapeutics 2004, 6, 191-200. 

Zhou, V. et al. 2004. A time-resolved fluorescence resonance energy transfer-based HTS assay and a surface plasmon resonance-based binding assay for heat shock protein 90 inhibitors. Anal. Biochem. 331, 349-357. 

T.L. Chew, W.A. Wolf, P.J. Gallagher, F. Matsumura, R.L. Chisholm,A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows. J. Cell Biol. 156(3), 543-553 (2002)... 

A. Hoppe, K. Christensen, J.A. Swanson, Fluorescence Resonance Energy Transfer-Based Stoichiometry in Living Cells, Biophys. J. 83, 3,652 (2002)... 

Imamura, H., Nhat, K.P., Togawa, H., Saito, K. et al. (2009) Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators. Proc. Natl. Acad. Sci. U.S.A., 106, 15651-15656. 

Kokko L, Kokko I, Lovgren T et al (2008) Particulate and soluble Eu(ni)-chelates as donor labels in homogeneous fluorescence resonance energy transfer based immunoassay. Anal Chim Acta 606 72-79... 

Kurokawa K, Mochizuki N, Ohba Y, Mizuno H, Miyawaki A, Matsuda M. A pair of fluorescent resonance energy transfer-based probes for tyrosine phosphorylation of the Crkll adaptor protein in vivo. J Biol Chem 2001 276 31305-31310. 

Kaufmaim, A. M. Goldman, S. D. B. Krise, J. P. A fluorescence resonance energy transfer-based approach for investigating late endosome-lysosome... 

Takakusa H, Kikuchi K, Urano Y, Kojima H, Nagano T (2003) A novel design method of ratiometric fluorescent probes based on fluorescence resonance energy transfer switching by spectral overlap integral. Chemistry 9 1479-1485... 

Takakusa, H., Kikuchi, K., Urano, Y., Sakamoto, S., Yamaguchi, K. and Nagano, T. (2002). Design and synthesis of an enzyme-cleavable sensor molecule for phosphodiesterase activity based on fluorescence resonance energy transfer. J. Am. Chem. Soc. 124, 1653-1657. 

Blagoi, G., Rosenzweig, N. and Rosenzweig, Z. (2005). Design, synthesis, and application of particle-based fluorescence resonance energy transfer sensors for carbohydrates and glycoproteins. Anal. Chem. 77, 393-399. 

The term filterFRET here refers to intensity-based methods for calculating fluorescence resonance energy transfer (FRET) from sets of images of the preparation collected at different excitation and/or emission wavelength. The term is not intended to imply that interference filters are actually present in the setup very similar considerations apply when donor- and acceptor fluorophores are spectrally resolved by other means, such as monochromators or spectral detectors. 

The upgrade of a frequency-domain fluorescence lifetime imaging microscope (FLIM) to a prismless objective-based total internal reflection-FLIM (TIR-FLIM) system is described. By off-axis coupling of the intensity-modulated laser from a fiber and using a high numerical aperture oil objective, TIR-FLIM can be readily achieved. The usefulness of the technique is demonstrated by a fluorescence resonance energy transfer study of Annexin A4 relocation and two-dimensional crystal formation near the plasma membrane of cultured mammalian cells. Possible future applications and comparison to other techniques are discussed. 

Zheng, J. (2006). Spectroscopy-based quantitative fluorescence resonance energy transfer analysis. Methods Mol. Biol. 337, 65-77. 

Fluorescence resonance energy transfer (FRET) has also been used very often to design optical sensors. In this case, the sensitive layer contains the fluorophore and an analyte-sensitive dye, the absorption band of which overlaps significantly with the emission of the former. Reversible interaction of the absorber with the analyte species (e.g. the sample acidity, chloride, cations, anions,...) leads to a variation of the absorption band so that the efficiency of energy transfer from the fluorophore changes36 In this way, both emission intensity- and lifetime-based sensors may be fabricated. 

The sensor for the measurement of high levels of CO2 in gas phase was developed, as well90. It was based on fluorescence resonance energy transfer between 0 long-lifetime ruthenium polypyridyl complex and the pH-active disazo dye Sudan III. The donor luminophore and the acceptor dye were both immobilized in a hydrophobic silica sol-gel/ethyl cellulose hybrid matrix. The sensor exhibited a fast and reversible response to carbon dioxide over a wide range of concentrations. 

Clapp AR et al (2007) Two-Photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications. Adv Mater 19 1921-1926... 

Wang X, Guo X (2009) Ultrasensitive Pb2+ detection based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles. Analyst 134 1348-1354... 

Clapp AR, Medintz IL, Uyeda HT, Fisher BR, Goldman ER, Bawendi MG, Mattoussi H (2005) Quantum dot-based multiplexed fluorescence resonance energy transfer. J Am Chem Soc 127 18212-18221... 

Medintz IL, Goldman ER, Lassman ME, Mauro JM (2003) A fluorescence resonance energy transfer sensor based on maltose binding protein. Bioconjug Chem 14 909-918... 

What mechanisms can be used to create a lifetime-based glucose sensor In our opinion, the mechanism should be fluorescence resonance energy transfer (FRET). The phenomenon of FRET results in transfer of the excitation from a donor fluorophore to an acceptor chromophore, which need not itself be fluorescent. FRET is a through-space interactor which occurs over distances of 20-60 A. 

Sensing Based on Fluorescence Resonance Energy Transfer (FRET)... 

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