Fluorescence resonance energy time-resolved measurements

Laitala V, Hemmila L. Homogeneous assay based on low quan- 83. turn yield Sm(lll)-donor and anti-Stokes shift time-resolved fluorescence resonance energy-transfer measurement. Analyt. Chim. 

Fluorescence recovery after photobleaching (FRAP) and the measurement of the fluorescence resonance energy (FRET), which allow the diffu-sional mobility of cellular components and their interaction at the molecular level to be monitored are other varieties of time-resolved methods [30]. A novel method is fluorescence correlation spectroscopy (FCS), which measures the statistical fluctuations of fluorescence intensity within a con-focally illuminated volume. Correlation analysis allows the concentration of particles and their diffusion to be determined [31], [32], FCS has proved... 

Leifert, W. Bailey, K. Cooper, T Aloia, A. Glatz, R. McMurchie, E. measurement of heteromeric G-protein and regulators of G-protein signaling interactions by time-resolved fluorescence resonance energy transfer. Anal. Biochem. 2006, 355, 201—212. 

Time resolved fluorescence measurements have become an important tool in applied fluorescence spectroscopy. Recently, it has been pointed out that the controlled manipulation of fluorescence decay rates opens a new dimension in applied fluorescence spectroscopy. The fluorescence decay rate depends on two independent contributions, the pure rachative rate and the nonradiative rate. The latter one can be influenced by the well known Forster-type resonant energy transfer processes, while the radiative rate can be changed if the molecules are embedded or close to media comprising a dielectric constant markedly different from vacuum. Especially metal nanostructures have been used to alter both decay paths of fluorescent molecules. Apart from a change of those two rates, the absorption cross-section might also be altered. 

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