Fluorescence experimental setup

In Refs. 20 and 21, an optical MNF was suggested and demonstrated as a sensor of atomic fluorescence. The experimental setup used in Ref. 21 is illustrated in... 

Figure 3 Experimental setup for single-molecule fluorescence imaging. The inset shows the molecular structure and the size of a rhB molecule. (From Ref. 16.)...

Fig. 2 A schematic view of an experimental setup for measuring the dynamical variable r(t). A single macromolecule is subjected to a train of short pulses with a repetition rate kex. r(tj) is defined as the time interval between photoexcitation of the donor at time tj and the emission of a fluorescence photon by either the donor (green) or the acceptor (red).

FIGURE 3.40 (a) Configuration of the experimental setup and white light microscopy image of an imprinted T-channel with a series of ablated wells, (b) Fluorescence images of electroosmotic flow past the mixer at flow rates of 0.06 cm/s [193]. Reprinted with permission from the American Chemical Society. 

To clarify the mutual interactions between the gas bubbles and its surrounding liquid flow (mostly turbulent) in a bubbly flow, information of bubble s shape and motion is one of the key issues as well as the surrounding liquid velocity distribution. Tokuhiro et al. (1998, 1999) enhanced the PIV/LIF combination technique proposed by Philip et al. (1994) with supplementation of SIT to simultaneously measure the turbulent flow velocity distribution in liquid phase around the gas bubble(s) and the bubble s shape and motion in a downward flow in a vertical square channel. The typical experimental setup of the combination of PIV, LIF, and SIT is shown in Figure 14. The hybrid measurement system consists of two CCD cameras one for PIV/LIF (rear camera) and the other for SIT (front). The fluorescent particles are Rhodamine-B impregnated, nominally 1-10 pm in diameter with specific density of 1.02, and illuminated in a light sheet of approximately 1 mm thickness (Tokuhiro et al., 1998,1999). The fluorescence is recorded through a color filter (to cut reflections) by the rear camera. A shadow of the gas bubble is produced from infrared LEDs located behind the gas bubble. A square "window" set within the array of LEDs provides optical access for... 

Experimental Setup. The instrumentation (both optics and electronics) for studying saturated laser induced fluorescence spectroscopy is much less conplicated than for CARS. The experimental setup shown in Figure 18, as used in our laboratory, is typical for these studies. In some experiments it is advantageous to use a monochromator rather than band pass filters to isolate the laser induced fluorescence signal. The lasers used are either flash lamp pumped systems or NdsYAG pumped dye lasers. 

A variety of experimental setups were developed for structure analysis of proteins, based on the excitation of the tryptophan moiety (the most brightly fluorescent proteogenic amino acid residue), that produces an intrinsic fluorescence emission of a folded protein. Tryptophane residues excited at wavelength values around 280-290 nm emit at a characteristic wavelength range (330-350 nm) thus reporting on how much this residue is buried within the protein. Techniques such... 

Figure 5. (A) Scheme of two-photon laser scanning microscope (1) Ti Sa laser, 100 fs, 80 MHz, 750-980 nm, 1.6W 800 nm (TSUNAMI, Spectra Physics), (2) pre-chirp, (3) beam multiplexer, (4) scanning mirrors, (5) microscope (Olympus IX 71, XLUMPLFL20XW, WD = 2 mm, NA = 0.95), (6) fluorescent foci in sample, (7) filter wheel/spectrograph (SpectraPro 2300i, Acton Research Corporation)/spectrometer (home built), (8) back illuminated EMCCD camera (IXON BV887ECS-UVB, Andor Technology), (9) dichroic mirror (2P-Beamsplitter 680 DCSPXR, Chroma). (B) Experimental setup of two-photon laser scanning microscope.

The rotational reorientation times of the sample in several solvents at room temperature were measured by picosecond time-resolved fluorescence and absorption depolarization spectroscopy. Details of our experimental setups were described elsewhere. For the time-correlated single photon counting measurement of which the response time is a ut 40 ps, the sample solution was excited with a second harmonics of a femtosecond Ti sapphire laser (370 nm) and the fluorescence polarized parallel and perpendicular to the direction of the excitation pulse polarization as well as the magic angle one were monitored. The second harmonics of the rhodamine-640 dye laser (313 nm 10 ps FWHM) was used to raesisure the polarized transient absorption spectra. The synthesis of the sample is given elsewhere. All the solvents of spectro-grade were used without further purification. 

Instrumental application of surface-plasmon-enhanced fluorescence was applied in using a TP scanning tunneling microscope [363], This was employed to probe the TP excited fluorescence from organic nanoparticles adsorbed on a silver surface. A size dependence of fluorescence enhancement and photodecomposition was reported as a result of competition between surface-plasmon-enhanced TP fluorescence and nonradiative energy transfer from the excited dye molecules to the silver surface. The schematic experimental setup is shown in Figure 3.14 [363]. 

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