FLP recombinase

Flp/FRT is a system analogous to the cre/loxP system. Flp is an yeast enzyme that recognizes FRT sites. If two FRT sites have a parallel orientation, the DNA segment between these sites will be deleted by the action of the Flp recombinase. 

Zhu XD, Sadowski PD (1995) Cleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic amino acid. J Biol Chem 270 23044-23054... 

Lee CL, Moding EJ, Huang X et al (2012) Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice. Dis Model Mech. doi 10.1242/dmm.009084... 

Microorganisms sometimes control the synthesis of surface proteins using segments of invertible DNA. The pathogenic bacterium Campylobacter fetus utilizes DNA rearrangements to allow one of a large family of surface layer (S-layer) proteins to be formed.597 The yeast FLP recombinase, mentioned in the preceding section, also inverts the sequence flanked by the 599-bp repeats.589... 

Y. Chen, U. Narendra, L.E. lype, M.M. Cox, and P. A. Rice. 2000. Crystal structure of a Flp recombinase-Holliday junction complex Assembly of an active oligomer by helix swappingMo/. Cell 6 885-897. (PuhMed)... 

FLP recombinase In vivo recombination efifirienry at elevated temperatures in E.coli and mammalian cells in vitro thermostability Improved recombination efficiency in E. coli and mammalian cells... 

Yeast FLP recombinase, acting upon FRT sequences inserted near the centromere, can be used to create clones of homozygous mutant cells in flies that are heterozygous for a recessive mutation. Recombinase is produced at a specified time using a heat-inducible promoter. Just enough recombinase is induced to cause an occasional recombination event. The effects of lost gene function are then assessed. 

When a modest pulse of FLP recombinase is produced using a heat-inducible promoter active in all cells, enough recombinase is made in a few cells to act on FRT sites and cause a deletion of the DNA between the FRTs. This removes the /acZgene at the same time that it joins a promoter to the gene of interest. The resulting clone of cells that expresses the gene of interest can be identified because the cells do not express lacZ. The effect of the gene of interest is then assessed in the clone. 

J.A. Sawicki, B. Monks, R.J. Morris, Cell-specific ecdysone-inducible expression of FLP recombinase in mammalian cells, Biotechniques 1998, 25, 868-870, 872-865. 

Fedoryshyn, M., Petzke, L., Welle, E., Bechtholi A. et al (2008) Marker removal from actinomycetes genome using Flp recombinase. Gene, 419 (1-2), 43-47. 

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