Fixed bed culture

Fassnacht D, Porter R (1999), Experimental and theoretical considerations on the oxygen supply of animal fixed-bed culture, J. Biotechnol. 72 169-184. 

Example of fixed-bed culture system in different modes of operation... 

Fig. 4. Schematic of a fixed-bed process for culturing mammalian cell using ceramic matrix.

The models proposed by Wu et al. [36] and by Lin and Leu [45] refer to continuous conversion processes by immobilized bacteria the first to a fixed mixed culture entrapped into PVA beads operated in a fluidized bed, and the second to BAC of P. luteola operated in a packed bed. Results of these models highlight the role of mass transport phenomena and biophase granule size on reactor performance. 

Gisi D, Stncki G, Hanselmann K W (1997) Biodegradation of the pesticide 4,6-dinitro-w//io-cresol by microorganisms in batch cultures and in fixed-bed column reactors, Applied Microbiology Biotechnology 48 441-448. 

Gibberellic acid production was studied in different fermentation systems. Free and immobilized cells of Gibberella fujikuroi cultures in shake-flask, stirred and fixed-bed reactors were evaluated for the production of gibberellic acid (GA3). Gibberellic add production with free cells cultured in a stirred reactor reached 0.206 g/L and a yield of 0.078 g of GA3/g biomass. 

Fig. 5. Gibberellic acid production with immobilized cells of G.fujikuroi cultured in fixed-bed reactor.

The fixed-bed, porous-glass-sphere culture system was designed for the production of secreted cell products and lytic virus. The system is based on the immobilization of cells (anchorage-dependent or suspension) to high cell densities in porous glass spheres (supplied by Schott Glaswerke). 

Figure 5.9.3 Schematic diagram of fixed-bed porous-glass-sphere culture system (1) packed bed of porous glass spheres (2) sampling port (3) medium fed in (4) air/oxygen sparge (5) off-gas filters (6) pH probe (7) dissolved oxygen probe (8) peristaltic pump (9) inoculation port (10) harvest.

SUPPLEMENTARY PROCEDURE ANALYSIS OF CONSUMPTION AND PRODUCTION RATES IN THE FIXED-BED POROUS-GLASS-SPHERE CULTURE SYSTEM... 

The productivities of the different culture systems are compared in Table 5.9.6, with reference to viable cell numbers and monoclonal antibody productivity. Table 5.9.7 gives a prediction of the scale-up of hybridoma growth and productivity in fixed-bed Siran porous-glass-sphere reactors. 

Racher AJ, Looby D Griffiths JB (1990) Studies on monoclonal antibody production of a hybridoma cell line (C1E3) immobilised in a fixed bed porosphere culture system. Journal of Biotechnology 15 129-146. 

For similar reasons as mentioned in denitrification, immobilized microorganisms would have advantages over suspended cultures in containing accumulated metals. In some cases, as it will be shown below, heavy metal uptake and some other microbiological functions, such as denitrification, can be performed in one operation, using the same fixed-bed bloreactor. 

Degradation of organopollutants by fungal cultures has been carried out successfully at bench and pilot scale, preferentially in batch or semi-continuous operations (Alleman et al. 1995 Fujita et al. 2002 Mielgo et al. 2002). Continuous treatment systems are desirable alternatives to increase process efficiency. A modified conventional configuration (fixed-bed bioreactor) coupled with a pulsation system has... 

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