Porous Microcarrier and Fixed-bed Cultures

The protocols for using the Verax System One are supphed with the culture unit and have been published previously (Looby, 1993). The culture productivity data presented in Tables 5.9.1 and 5.9.2 demonstrate its capabihty in comparison with other systems and during scale-up. 

An alternative commercial system that is now available is the Cytopilot (Pharmacia) (Reiter et al, 1990, 1991), which is a 25-1 system using polyethylene carriers (Cytoline) that supports up to 1.2 x 10 CHO K1 cells mH carrier. The range of porous microcarriers available is given in Table 5.9.3. 

Cell and Tissue Culture Laboratory Procedures in Biotechnology, edited by A. Doyle and J.B. Griffiths. 1998 John Wiley Sons Ltd. 

System Max. cell number viable (xlQio Monoclonal antibody productivity average (mg 1-1 dar f 

System Cell numbers (xl0 viable) Steady-state Bed volume productivity fluidized (1) (g dar ) Medium feed rate (1 dayi) 

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