Gibberella fujikuroi

Fig. 3. Preferential deposition of water insoluble pigment bikaverin (dark) at the filament branching points in the fungus Gibberella fujikuroi.

Thippeswamy, S. and Mulimani, V.H., Enzymic degradation of raffinose family oligosaccharides in soymilk by immobilized alpha-galactosidase from Gibberella fujikuroi, Proc. Biochem., 38 (2002) 635-640. 

Leslie, J. F. (1995). Gibberella fujikuroi Available populations and variable traits. Can. ]. Bot. 73 (Suppl. 1), S281-S291. 

O Donnell, K., Cigelnik, E., and Nirenberg, H. 1. (1998). Molecular systematics and phylo-geography of the Gibberella fujikuroi species complex. Mycologia 90,465 93. 

ATP -h trans-farnesyl diphosphate <1> (<1> possibly involved in control of biosynthesis of terpenoids in Gibberella fujikuroi [2]) (Reversibility <1> [2]) [2]... 

Shechter, I. Phosphate transfer from trans-farnesyl triphosphate to AMP in Gibberella fujikuroi. Biochim. Biophys. Acta, 362, 233-244 (1974)... 

Brian, P.W., Elson, G.W., Hemming, H.G., Radley, M. (1955). The growth promoting properties of gibberellic acid, a metabolic product of the fungus Gibberella fujikuroi. J. Sci. Food Agr. 5, 602-612. 

Microbiological Methods. The low substrate specificity of many of the enzymes involved in GA biosynthesis in Gibberella fujikuroi has been utilized for the preparation of higher plant GAs. Suitable analogs of the natural GA-precursors are converted by the fungus to the corresponding GA analogs. It is usual to prevent the synthesis of the natural GAs in order to facilitate purification of the unnatural products. A mutant strain, Bl- la, in which GA biosynthesis is blocked early in the pathway (67) (between ent-kaurenal and ent-kaurenoic acid) has been used. 

Gibberellin Biosynthesis in the Fungus Gibberella fujikuroi and in Higher Plants... 

Figure 2. The GA biosynthetic pathway from, MVA to GAlsraldehyde. This pathway is found in the fungus Gibberella fujikuroi and higher plants...

Bearder, J.R., MacMillan, J., Weis, C.M., Chaffey, M.B., Phinney, B.O. "Position of the metabolic block for gibberellin biosynthesis in mutant Bl-Ula of Gibberella fujikuroi." Phytochemistry, 19lh, 13, 911-917. 

Evans, R., Hanson, J.R. "Studies In terpenoid biosynthesis. Part XIII. The biosynthetic relationship of the gibberellins in Gibberella fujikuroi." J.C.S. Perkin Trans., 1975 1 ... 

Harrison, D.M., MacMillan, J. "Two new gibberellins, Ag4 and A25 from Gibberella fujikuroi their isolation, structure, and correlations with gibberellin A13 and Ane." J. Chem. 

ABA formation that cannot be readily conducted with higher plants. Similar studies with the fungus Gibberella fujikuroi, which produces gibberellins, have greatly advanced our knowledge of this group of hormones as reported by Fhinney in this Symposium. 

Gibberellic acid production was studied in different fermentation systems. Free and immobilized cells of Gibberella fujikuroi cultures in shake-flask, stirred and fixed-bed reactors were evaluated for the production of gibberellic acid (GA3). Gibberellic add production with free cells cultured in a stirred reactor reached 0.206 g/L and a yield of 0.078 g of GA3/g biomass. 

Index Entries Cell immobilization gibberellic acid Gibberella fujikuroi shake flasks free cells immobilized cells. 

Riboflavin is produced by Clostridium, Ascomycetes and Candida species. The yield can be as high as 5 g 1 1 after 7 days 60). Gibberella fujikuroi is utilized for the synthesis of gibberellins, a group of plant hormones used for plant growth promotion. Glucose, molasses, lipid (corn oil) are usually used as carbon sources. Vitamin B12 may also be synthesized from alcohols and hydrocarbons. 

The biosynthesis of GAs was extensively surveyed during the 1960-70s by feeding experiments using Gibberella fujikuroi and radioactive precursors.199 200 The studies on the biosynthesis of GAs in plants were led by Graebe and coworkers.201-203 Recent research progress in the biosynthesis of GAs is described in Section 4.02.3.4. 

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