Final copy, removal

The cloning procedure involves three basic steps. First, a donor cell is obtained from an animal that is to be cloned. Next, an egg cell is obtained from an animal that will act as the surrogate mother for the cloned animal. The DNA-containing nucleus of the egg cell is removed and replaced with the nucleus (and the DNA) from the donor cell of the animal being cloned. Finally, the modified egg cell is re-implanted in the surrogate mother, and the embryo, which now contains DNA identical to that of the donor animal, develops. The resulting cloned animal is an exact genetic copy of the donor animal and has all the properties, characteristics, etc., of the donor animal. 

A typical waste label that might be used at any facility to identify and document waste internally was shown in Figure 4.13 earlier. A form such as this should be attached to each container of surplus chmical before it is removed from the laboratory and should remain with the container until final disposition of the material. A copy should be retained by the waste facihty as part of their records. There are two sections to this form, one identifying the source of the material and the other characterizing the material itself The latter information is to be used by the personnel responsible for classifying the material, and if necessary to complete the preparations for its disposal as a hazardous waste. Note that the material is not classified as a hazardous waste, allowing it to be evaluated, perhaps as a usable or recyclable material. 

Solvent-exchange IPC samples are usually analyzed by direct injection capillary GC with flame ionization detection (FID) NIR, and Raman spectros-copy have also been used for this assay. The final acceptance criteria are typically expressed in % volume/volume (%, v/v) or % weight/volume (%, w/v) of the solvent being removed relative to the volume of solvent in the process mixture. 

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