Filamentous bacteriophages

In a multidomain protein whose domains have fixed orientations relative to each other, a unique alignment tensor will represent the preferred orientation of all the domains in the anisotropic environment. Therefore, structure refinement with dipolar couplings is performed as in one-domain proteins (Sect. 8.4). Several examples are reported in the literature of cases with conformational ambiguity due to the lack of NOE contacts between the domains. One example is the determination of subdomain orientation of the riboso-mal protein S4 z)41 [97]. In this work the lack of NOE contacts between the domains produces an ambiguity in interdomain orientation. The authors use two different anisotropic media to obtain dipolar couplings (DMPC/DHPC bicelles and Pfl filamentous bacteriophages). They conclude that subdomain orientation in solution is similar to the one present in the crystal structure. 

Greenwood J, Hunter GJ, Perham RN. Regulation of filamentous bacteriophage length by modification of electrostatic interactions between coat protein and DNA. J Mol Biol 1991 217(2) 223-227. 

Poul MA, et al. Targeted gene delivery to mammalian cells by filamentous bacteriophage. J Mol Biol 1999 288(2) 203-211. 

Filamentous bacteriophages. Bacteriophages of the Ff family include the fd, fl, and M13 strains.313,32-36 Phage Ml 3 is widely used in cloning genes and for many other purposes (Chapter 26). The genome is a circular, single-stranded DNA of 6400 nucleotides... 

Filamentous bacteriophage can only be propagated in E coli host strains that produce F -plasmid encoded sex pill... 

Makowski, L. 1994. Phage display structure, assembly and engineering of filamentous bacteriophage. ( hit. Opin. Struct. Biol. 4, 225—230. 

The E. coli filamentous bacteriophages, such as M13, fd, and fl, are the most commonly used viruses in biological display systems. Their genomes are small... 

Sampath A, Abrol S, Chaudhary VK, Versatile vectors for direct cloning and ligation-independent cloning of PCR-amplified fragments for surface display on filamentous bacteriophages, Gene, 190 5-10, 1997. 

Soumillon P, Jespers L, Bouchet M, Marchand-Btynaert J, Winter G, Fastrez I, Selection of beta-lactamase on filamentous bacteriophage by catalytic activity, I. Mol. Biol., 237 415 422, 1994. 

FIGURE 17.2 Genome of wild-type filamentous bacteriophage M13. Numbers correspond to different genes coding for 10 different viral proteins. IG intergenic space ori origins of replication for the + and — strands. 

Greenwood J, Willis AE, Perham RN, Multiple display foreign peptides on a filamentous bacteriophage Peptides from Plasmodium falciparum circumsporozoite protein as antigens, J. Mol. Biol., 220 821-827, 1991. 

Holliger P, Riechmann L, A conserved infection pathway for filamentous bacteriophages is suggested by the structure of the membrane penetration domain of the minor coat protein g3p from phage fd, Structure, 5 265-275, 1997. 

McGregor D, Selection of proteins and peptides from libraries displayed on filamentous bacteriophage, Mol. Biotechnol., 6(2) 155—162, 1996. 

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