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Ligation-independent cloning

Haun, R. S., Serventi, I. M. and Moss, J. (1992). Rapid, reUable ligation-independent cloning of PCR products using modified plasmid vectors. Biotechniques 13, 515-518. [Pg.42]

Stols, L., Gu, M., Dieckman, L., Raffen, R., CoUart, F. R. and Donnelly, M. 1. (2002). Anew vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site. Protein Expr. Purif. 25, 8-15. [Pg.43]

Key Words Ligation-independent cloning T7 expression system pET vector hexa-histidine tag. [Pg.107]

Construction of a Ligation-Independent Cloning-Compatible Plasmid... [Pg.108]

Fig 2. Ligation-independent cloning (LIC) using the pNHis vector. The bold type denotes the LIC sequences of the vector, which contains the BseR I recognition sequence (underlined). The complementary LIC sequences of the insert DNA are shown in italics. [Pg.110]

Aslanidis, C., de Jong, P. J., and Schmitz, G. (1994) Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products. PCR Methods Appl. 4(3), 172-177. [Pg.113]

Sampath A, Abrol S, Chaudhary VK, Versatile vectors for direct cloning and ligation-independent cloning of PCR-amplified fragments for surface display on filamentous bacteriophages, Gene, 190 5-10, 1997. [Pg.406]

LIC-PCR ligation-independent cloning-polymerase chain reaction... [Pg.714]

SLIC A Method for Sequence- and Ligation-Independent Cloning... [Pg.51]

Key words SLIC, Ligation-independent cloning. In vitro homologous recombination, iPCR,... [Pg.51]

S.Aslanidis C, de long PI (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res 18 6069-6074... [Pg.59]

See other pages where Ligation-independent cloning is mentioned: [Pg.105]    [Pg.114]    [Pg.23]    [Pg.24]    [Pg.24]    [Pg.24]    [Pg.40]    [Pg.41]    [Pg.107]    [Pg.107]    [Pg.111]    [Pg.113]    [Pg.450]    [Pg.695]    [Pg.702]    [Pg.705]    [Pg.705]    [Pg.40]    [Pg.763]    [Pg.138]    [Pg.141]    [Pg.174]    [Pg.192]    [Pg.51]    [Pg.51]   


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