Ferric chloride testing

Ferric chloride test. Dissolve 1 drop or 0 05 g. of the compound in 5 ml. of water and add 1 drop of ferric chloride solution observe the colour produced. If the result is negative in aqueous solution, repeat the test in alcoholic solution. 

The mixture was refluxed gently on a steam bath for VA hours. Fifteen minutes after initiating the reaction, the reaction mixture gave a negative ferric chloride test. Most of the ethanol and acetic acid were removed by distillation in vacuo, 300 ml of water and 300 ml of ether were added to the concentrate, and the mixture was shaken. The layers were separated, the aqueous layer extracted with fresh ether, and the combined ether extracts were washed with water, dried over anhydrous sodium sulfate, filtered and evaporated to dryness in vacuo. The residue was crystalli2ed by trituration with ether, and the crystals were collected by filtration, washed with hexane and dried. The mother liquors were concentrated to dryness and dissolved in a minimum amount of acetone, whereupon a second crop was obtained. The two crops were combined, dissolved in ethyl acetate, decolori2ed with activated charcoal, and recovered by concentration. 

Ferric chloride test Add 10 drops of 3% aqueous FeCl3 solution to 1 ml of a 5% aqueous (ethanol) solution of the compound in question. Phenols give red, blue, purple, or green colorations. The same test can be done by using chloroform as a solvent (detection limit, 50 pg). 

Phenols Acetylation Benzoylation Sulfonation Ferric chloride test Solubility in aqueous base ppt of a characteristic melting point ppt of a characteristic melting point ppt of a characteristic melting point Variety of colors characteristic of the individual phenol Most phenols are soluble in dilute sodium hydroxide but insoluble in dilute sodium bicarbonate phenols with strong electron withdrawing groups (e.g., picric acid) are soluble in sodium bicarbonate... 

Hydrolysis of deferriferricrocin in 57% HI gives serine, glycine, alanine, ornithine and ammonia in the molar ratios 0.25 2.00 0.68 3.05 0.04 no alanine is found on hydrolysis in 6N HCI. Treatment with IN HCI at 100° for 15 min. gives substance with negative ferric chloride test. Re-acetylation with acetic anhydride and removal of O-acetyl affords a product with chromatographic properties of ferricrocin. 

A variety of modifications can be made to the ferric chloride test and still... 

Phenothiazines can cause increased urinary ketones (ferric chloride test) (SED-11,115). 

Ferric ammonium sulphate solution, 1169 Ferric chloride solution, 1169 Ferric Chloride (test), 133 Ferric iron, test on stomach contents, 5 Ferricyanide test on stomach contents, 5 Ferrioxamine, 516... 

Data Sheet 13.2 Ferric chloride test for phenolics Results for some deciduous and coniferous trees... 

The ferric chloride test is negative, and the filtrate remains colorless when hydrogenation is complete. A deep blue color (due to the presence of gallic acid or dihydro products) appears when it is not, but the hexahydro acid can usually be isolated in good yield in spite of this. 

Note that 2- or 3-methyl-2-butcne-l-ols are the chain terminal units of isoprenoids. Demethylation. [>emcthylation of 4,5-dimethoxybenzocyclobutene-l,2-dionc (1) was effected by treatment with 48% hydrobrutnic acid. 4,5-Dimcthoxybenzocyclo-butene-l,2-dione is a benzolog of squaric acid. It gives a positive ferric chloride test. 

Tests for Pitting. A test which is in popular use to determine pitting characteristics is the 10 percent Ferric Chloride test which can be conducted at room temperature. This test is usually used for stainless or alloy steels. 

Doering and Odum found that removal of by-product phenol increases the stability of phenyl azide in storage. Thus the ethereal extract of the steam distillate was extracted with IN sodium hydroxide until no phenol could be detected by the ferric chloride test. Concentration and vacuum distillation afforded phenyl azide in 31% yield. 

None of the commonly used methods for the detection and determination of ketone bodies in serum or urine reacts with ah three ketone bodies. Gerhardt s ferric chloride test reacts with acetoacetate only. Tests using nitroprusside are at least 10 times more sensitive to acetoacetate than to acetone, and give no reaction at all with P hydroxybutyrate. 

The chemical test most frequently used for determination of azides is the ferric chloride test. The azide ion forms a deep red complex with ferric ion, which is readily detected by the unaided eye. 

Vomicidine is phenolic, soluble in alkali, and capable of the ready oxidative destruction typical of o-aminophenols the fact that vomicine itself appears not to be phenolic, on the other hand (not acylatable or soluble in alkali no ferric chloride test), has been ascribed to a masking of the hydroxyl by internal attack on the lactam carbonyl (CIX) (336). Another explanation is that the hydroxyl is just very strongly... 

This compound, m.p. 196-198°, was isolated from the corms of C. autumnale (294). No analytical data were presented, but a tropolone ring was indicated by a positive ferric chloride test after hydrolysis with dilute acid. 

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