Feeding assay

The defensive furanocembranes from the adult soft coral Sinularia poly-dactyla are also found in the eggs and larvae of their offspring. HPLC-com-parison of extracts from offspring with those from adults showed that pukalide (39) and 1 ip-acetoxypukalide (40) (Scheme 10) [87,88] are contained in both the blastula and planula of the soft coral [89]. Feeding assays with the larvae and their extracts showed that fish rejected the larvae due to chemical factors -most likely the pukalides. The pukalide-concentrations in the extracts of... 

Neither 79 nor 80 are detected in the intact tissue of the sponge implying that substrates and enzymes for the conversion are stored in different cellular compartments or different cell types. When isofistularin-3 (76) is the substrate, 77 is detected as a second cleavage product of this reaction. This wound-activated bioconversion is paralleled by a marked increase of biological activity of the products 79 and 80 compared to the isoxazolines. In feeding assays the aqueous... 

From the flotation feed assaying 0.87% Sn, a concentrate grade assaying 10.6% Sn at 81.5% Sn recovery was achieved. Further upgrading of the concentrate was achieved by magnetic separation. 

The artificial diet feeding assay mentioned above was employed to monitor the chromatographic separation of the 5 bioactive principles. Once separated, purified, and spectrally identified, the active principles were synthesized and tested in the same artificial diet feeding assay in order to obtain ED5Q-values, the effective doses for 50% growth inhibition. 

Apoplastic transport, achieving, 197 Apparent partition coefficient, 227 Area, relationships, 260 Artificial diet feeding assay, 163-64 2-Ary1-1-cycloalkanediones biological properties, optimization, 321 structure-activity... 

Feeding assays Assays were performed with diets placed between two layers of Parafilm M (23). The diet was as described (24). When young aphids were used (survival and feeding deterrence assays) they were 3rd and 4th-instar nymphs. 

FIGURE 11.3 Levels of screen automation, (a) Workstation-based automation can be categorized as (1) batch automation in which plate stackers feed assay plates into each device and plate stacks are transported between devices manually and (2) automated workstations that include liquid dispensers and readers and can transfer plates between the two devices automatically, (b) In a fully automated system, transport of plates between devices is carried out by robotic arms and scheduling software. 

Recent studies have examined the chemical basis of sea hare response to attack and have identified amino acid constituents in the opaline glands and ink secretions of A. californica that stimulate a false feeding response ( phagomimicry ) as well as confused behavior in the shiny lobster (Panulirus interruptus) 194-195 Both ink and opaline secretions are acidic, believed to enhance behavioral responses.196 The importance of inking as a deterrent mechanism is revealed by feeding assays in which reef fish consumed frozen mollusks but did not eat live specimens.43... 

As a result, we isolated only abyssinin as an insect growth inhibitor while monitoring by an artificial diet feeding assay. 

Maltby, L., Clayton, S.A., Wood, R.M. and McLoughlin, N. (2002). Evaluation of the Gammarus pulex in situ feeding assay as a biomonitor of water quality robustness, responsiveness, and reliance. Environ. Toxicol. Chem., 21, 361-368. 

Mortality in first instar M. sexta larvae is determined in a six day feeding assay... 

Use feed assay and liberation size as criteria to guide selection of options. 

---