Fast HPLC analysis

ON-LINE COUPLING OF SPE-HPLC-MS/MS FOR FAST TRACE ANALYSIS OF PESTICIDES IN DRINKING AND SURFACE... 

Chromatographic Characterization of TTXs. The vast majority of reports have identified TTX and anhydro-TTX in bacterial cultures using HPLC, TLC, and GC-MS. Yasumoto et al. (30) showed that TTX-like substances extracted from a Pseudomonas sp. culture could bind to activated charcoal at pH 5.5 and be eluted with 20% ethanol in 1% acetic acid. In addition, HPLC analysis demonstrated TTX and anhydro-TTX-like fluorophors following strong base treatment. These compounds migrated on silica gel comparably to TTX and anhydro-TTX. Furthermore, when analyzed by electron ionization (EI)-MS and fast atom... 

Most modern HPLC instruments include a column oven that can thermostat the column to at least 100°C. A typical HPLC analysis can be done in half the time by elevating the column temperature from ambient to 50 or 60°C. At temperatures above 100°C, it is not uncommon to decrease analysis time by a factor of 5.26 Also, re-equilibration time for the column is much shorter, so it is possible to achieve ultra-fast gradient analysis with HTLC. 

More recently, attention has turned to the aftertreatment of commercially available mordant dyes on wool with iron(II) and iron(III) salts as a potential source reduction approach to eliminating chromium ions from dyebath effluent [34]- The anticipated improvements in fastness performance were achieved. The structures of the conventional 1 2 iron-dye complexes formed on the wool fibres were characterised by negative-ion fast-atom bombardment spectroscopy and HPLC analysis [35]. 

We focus the following discussion on analysis techniques for small molecular entities. The reader should be aware that there are also a number of solutions for analytical tools that are useful for fast HPLC, GPC, or even DSC. The classification principles that apply for these techniques are the same as for the analytical tools described in this chapter. 

Hsieh, Y. Brisson, J. M. Wang, G. Ng, K. Korfmacher, W. A. Simultaneous fast HPLC-MS/MS analysis of drug candidates and hydroxyl metabolites in plasma. 

In this unit, methods for reversed-phase high-performance liquid chromatography (HPLC) are described for the analysis of polyphenolics. HPLC analysis can be employed in an easy and fast manner to obtain an accurate elucidation and quantification of individual polyphenolic compounds found in plant-based materials. The separation of each polyphenolic is based on the polarity differences among polyphenolics with structural similarities and uses various combinations of mobile and stationary phases. 

SPME is a fast, universal, sensitive, solventless, and economical method of preparing samples for GC or HPLC analysis, enabling detection limits at a level of 5-50 ppt for volatile, semivolatile, and nonvolatile compounds to be achieved. The approximate sample preparation time is usually 2-15 min.49-50... 

The introduction of "fast HPLC" has proven to be particularly valuable in protein analysis. As stated earlier, assay time in RP-HPLC analysis of proteins is typically long compared to that for smaller organic molecules. We have evaluated the use of 0.6-cm ID x 4-cm columns packed with 3-um particles in the analysis of insulin by RP-HPLC for potency determination, related substances, and in peptide mapping (7). The use of the "fast column" allows considerable savings (40-60%) in analysis time, compared to the regular (0.46-cm ID x 25-cm) columns, without loss in resolving power. 

For this reason, the performance of these instruments is less satisfactory in applications with high speed columns which allow the analysis of biopolymers to be carried out on a time scale of a minute or less. In order to expand the scope of HPLC to such applications, some of the considerations described below should be taken in to account in the design of fast HPLC analyzers for biological macromolecules. 

Liquid chromatography can be used in real time to aid in kinetic studies. All that is required is that the reactant(s) and product(s) be separable. If the reaction is fast, the reaction must be quenched before the HPLC analysis or the analysis time and sampling time must be accounted for. 

Minano FJ, Peinado JM, Myers RD (1989) Neurotensin perfused in hypothalamus of sated or fasted rat HPLC analysis of release of DA, NE and 5-HT and their metabolites. Peptides 9 1381-1387. 

Koellensperger, G., Hann, S. Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine. Anal. Bioanal. Chem. 397, 401 06 (2010)... 

---