Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Fast-column HPLC, protein analysis

The introduction of "fast HPLC" has proven to be particularly valuable in protein analysis. As stated earlier, assay time in RP-HPLC analysis of proteins is typically long compared to that for smaller organic molecules. We have evaluated the use of 0.6-cm ID x 4-cm columns packed with 3-um particles in the analysis of insulin by RP-HPLC for potency determination, related substances, and in peptide mapping (7). The use of the "fast column" allows considerable savings (40-60%) in analysis time, compared to the regular (0.46-cm ID x 25-cm) columns, without loss in resolving power. [Pg.120]

A variant of HPLC is FPLC (fast protein liquid chromatography) (Sheehan 1996), a form of chromatography in which the apparatus and column material is designed specifically with protein analysis and purification in mind. However, this does not imply that conventional HPLC with the right columns is unsuitable for protein chromatography. [Pg.99]

See other pages where Fast-column HPLC, protein analysis is mentioned: [Pg.109]    [Pg.12]    [Pg.162]    [Pg.172]    [Pg.538]    [Pg.1197]    [Pg.18]    [Pg.366]    [Pg.174]    [Pg.2]    [Pg.257]    [Pg.113]    [Pg.132]    [Pg.167]    [Pg.728]    [Pg.728]    [Pg.203]    [Pg.639]    [Pg.396]    [Pg.80]    [Pg.137]    [Pg.639]   
See also in sourсe #XX -- [ Pg.120 ]



SEARCH



Column fast analysis

Fast HPLC

Fast HPLC analysis

Fast protein HPLC

Fast protein analysis

HPLC analysis

HPLC column

Protein analysis

Protein column

© 2024 chempedia.info