Extraction, in situ

Blinks used a different method to dislodge the particles of active material from the strips cut off from the jellyfish (Blinks et al., 1978). The strips are shaken in cold seawater, and the particles dislodged are harvested by filtration on a Buchner funnel with the aid of Celite. The filter cake is first washed with 50 mM EDTA, pH 8.0, containing (NH4)2S(>4 at 75% saturation, to remove seawater. Then, the particles are cytolyzed and aequorin is extracted in situ by washing the filter cake with cold 50 mM EDTA, pH 8.0. The filtrate is clear and slightly greenish. The active matter in the filtrate is precipitated by saturation... 

Mindevalli, O. and Pedram, E.O., Soil bioventing and vapor extraction (in-situ and ex-situ) and ground-water treatment and reinjection at a diesel and gasoline contaminated site, Proc. HAZMACON 94, Bursztynsky, T., Ed., 1994, pp. 386-398. 

Alternative 4 (In S fu Soil Vapor Extraction, In Situ Soil Fixation, Cap, Groundwater Pump-and-Treat... 

Cummings, J. (1999). Steam Enhanced Extraction - In Situ NAPL Recovery at the Southern California Edison Visalia Pole Yard, Presented at EPA Innovative Clean-up Approaches Conference, Indian Lakes Resort, Bloomingdale, IL November 2-4. 

Keywords. Plant cell culture, In situ extraction, In situ adsorption, Cyclodextrin, Aqueous two-phase systems... 

Integrated bioprocesses can be used to enhance the production of valuable metabolites from plant cell cultures. The in situ removal of product during cell cultivation facilitates the rapid recovery of volatile and unstable phytochemicals, avoids problems of cell toxicity and end-product inhibition, and enhances product secretion. In situ extraction, in situ adsorption, the utilization of cyclodextrin, and the application of aqueous two-phase systems have been proposed for the integration of cell growth and product recovery in a bioreactor. The simultaneous combination of elicitation, immobilization, permeabilization, and in situ recovery can promote this method of plant cell culture as a feasible method to produce various natural products including proteins. 

Gao, J., Garrison, A.W., Hoehamer, C., Mazur, C., and Wolfe, N.L., Phytotransformations of organophosphate pesticides using axenic plant tissue cultures and tissue enzyme extract. In situ and on-site bioremediation. The Fifth International Symposium, San Diego, 19-22 April 1999. 

Many compounds have now been used as template molecules in molecular imprinting. Basically, imprinted polymers can be used directly as separation media. Since all separation applications cannot be described here, some studies recently reported are bsted in Table 7.1. In this chapter, only selected topics, including sensor applications, signaling polymers, molecularly imprinted sorbent assays, molecularly imprinted membranes, affinity-based solid phase extraction, in situ preparation of imprinted polymers, and molecularly imprinted catalysts are discussed. For the reader requiring information on other applications, there are many review articles dealing with these, Recent review articles and books are summarized in Table 7.1. For further development of molecular imprinting techniques, newly designed functional monomers would be desirable. Various functional monomers have been reported and many applications have been conducted. These are summarized in Table 7.2. 

Methode M.403-PEST4.0 Eaux — Determination des pesticides de types organophosphores, triazines, carbamates, urees substituees, phtalimides et pyrethrinoides. Extraction in situ avec dichloromethane dosage par chromatographie en phase gazeuse couplee a un... 

A new promising sampling technique is described by Ehrhardt (1976) and Dawson et al. (1976), whereby the samples are extracted in situ without contamination or loss, using a glass column filled with a non-specific substrate lowered directly to the desired depth. 

These consecutive reactions can be avoided by using the biphasic method [10], which allows the selective production of the primary amines 4 and 5. The reaction takes place in an aqueous catalyst phase (Pd(OAc)2 + TPPTS) and the primary products are extracted in situ by a second organic phase (e.g., toluene, methylene chloride, butadiene) which is immiscible with water. 

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