Expanded-bed adsorption for protein purification

PHYSICOCHEMICAL BASIS OF EXPANDED-BED ADSORPTION FOR PROTEIN PURIFICATION... 

A number of applications are given in this chapter that detail the capture and recovery of intracellular proteins including recombinant proteins. The initial protein recovery steps, regardless of the source, are usually associated with large volumes and crude solutions, requiring removal of particles and reduction of volume before their purification can take place. Centrifugation, filtration, precipitation, solvent extraction, and batch adsorption are common unit operations involved in the preliminary steps of protein recovery. Expanded-bed adsorption, as described here, is an approach for the initial protein recovery that eliminates the need for clarification and volume reduction. In this process, a crude starting solution is pumped directly onto an... 

Chang, Y. K., and Chase, H. A. (1996). Development of operating conditions for protein purification using expanded bed techniques The effect of degree of bed expansion on adsorption performance. Biotechnol. Bioeng. 49, 204-216. 

STREAMLINE expanded bed adsorption is particularly suited for large scale recombinant protein and monoclonal antibody purification. STREAMLINE adsorbents are specially designed for use in STREAMLINE columns. The technique requires no sample clean up and so combines sample preparation and capture in a single step. As shown in Figure 10, crude sample is applied to an expanded bed of STREAMLINE media, target proteins are captured whilst cell debris, particulate matter, whole cells, and contaminants pass through. Flow is reversed and the target protein is desorbed in the elution buffer. 

Expanded bed adsorption was chosen because the target protein was captured directly from the crude homogenate in a single step, without the need for centrifugation or other preparatory clean-up steps. The technique is well suited for large scale purification. 

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