Examples of Antibody Dilutions

As an example, optimal dilutions of four antibodies commonly used for p53 protein in squamous cell carcinomas are given below (Piffko et al., 1995). 

Wild-type p53 protein is difficult to detect immunohistochemically in normal cells because of its very short half-life (—20 min) and its presence in minute amounts. However, wild-type p53 protein accumulation can be detected by using antigen retrieval techniques (Dowel and Ogden, 1996 Hall and Lane, 1994). On the other hand, because mutant p53 

FIGURE 10.7. Immunostained p53 antigen in the moderately differentiated invasive ductal carcinoma of the pancreas of a 54-year-oid female the majority of the nuclei are stained. Sections were microwaved and incubated in mouse monoclonal antibody DO-1. Courtesy of Ming Dong. 

TABLE 10.5. Immunohistochemical Localization of p53 Antigen in Various Carcinomas 

Non-smatl-cell lung cancer Schiller etal. (2001) 

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