Ethylenediaminetetraacetic blood samples

A 1-ml ethylenediaminetetraacetic acid (EDTA)-treated blood sample is sufficient for both analyses. Blood is usually transported to the laboratory within a day, at which point the cells are separated from the plasma. The latter is stored at - 20°C, whereas the red cells are stored at 6°C for a maximum of 3 weeks. Should the red... 

Plasma isolated from blood samples obtained by venipuncture and collected in standard heparin or ethylenediaminetetraacetic-acid-treated glass tubes prepared... 

The preferred blood sample is one collected with either K or Na salts of ethylenediaminetetraacetic acid (EDTA) as the anticoagulant. To minimize the formation of degradation products, which are especially noticeable as small bands eluting with similar retention time as Hb Ai and Hb F on high-performance liquid chromatography (HPLC) analysis, testing should be performed within 5 days of collection and samples should be stored at 4 "C. 

Pseudothrombocytopenia, defined as in vitro platelet aggregation in blood anticoagulated with ethylenediaminetetraacetic acid (EDTA), is clinically insignificant, but it must also be differentiated from thrombocytopenia induced by abciximab. In this case, microscopic examination of a peripheral blood smear, along with repeated platelet counts in citrate-anticoagulated blood samples, makes the distinction possible. ... 

If the analyte is very volatile (e.g., propane or butane) and a quantitative analysis is required, a blood sample should be collected directly into the headspace vial in which the analysis will be carried out. Many other volatile compounds are relatively stable in blood and other tissues if simple precautions are taken. In the case of blood, the container used for the sample should be glass, preferably with a cap lined with metal foil greater losses may occur if plastic containers are used. The tube should be as full as possible and should only be opened when required for analysis and then only when cold (4°C). If the sample volume is limited, it is advisable to select the container to match the volume of blood so that there is minimal headspace. An anticoagulant [sodium ethylenediaminetetraacetate (EDTA) or lithium heparin] should be used. Specimen storage between - 5 and 4°C is recommended and 1% (w/v)... 

Blood is collected in 2.7 ml ethylenediaminetetraacetic acid (EDTA) tubes (Sarstedt, Switzerland) containing 0.1% (w/v) dithioerythritol (DTE), immediately centrifuged at 2000 xg for 10 min, and stored at -80°C. Keep fresh or thawed plasma samples on ice during the oxidation procedure. 

The two major kinds of samples analyzed for xenobiotics exposure are blood and urine. Both of these sample types are analyzed for systemic xenobiotics, which are those that are transported in the body and metabolized in various tissues. Xenobiotic substances, their metabolites, and then-adducts are absorbed into the body and transported through it in the bloodstream. Therefore, blood is of unique importance as a sample for biological monitoring. Blood is not a simple sample to process, and subjects often object to the process of taking it. Upon collection, blood may be treated with an anticoagulant, usually a salt of ethylenediaminetetraacetic acid (EDTA), and processed for analysis as whole blood. It may also be allowed to clot and be centrifuged to remove solids the liquid remaining is blood serum. 

Sample preparation Plasma. 1.5 mL Blood + 50 xL 100 mM ascorbic acid and 100 mM disodium ethylenediaminetetraacetate, centrifuge. Remove 0.5 mL plasma and immediately add it to 50 (jiL 1 mg/mL p-bromophenacyl bromide, vortex 30 s, let stand at room temperature for 20 min, add 300 p.L 6% perchloric acid, vortex for 30 s, centrifuge at 10000 g for 10 min, inject a 500 j,L aliquot onto column A with mobile phase A and elute to waste, after 3 min elute the contents of column A onto column B with mobile phase B, after 2 min remove column A, elute column B with mobile phase B, monitor the effluent from column B. Urine. 50 p,L Urine + 50 jiL 1 mg/mL p-bromophenacyl bromide + 600 pL water, vortex 30 s, let stemd at room temperature for 20 min, inject a 500 p.L aliquot onto column A with mobile phase A and elute to waste, after 3 min elute the contents of column A onto coliunn B with mobile phase B, after 2 min remove column A, elute coliunn B with mobile phase B, monitor the effluent from column B. (Colmnn A should be washed with MeOH for 2 min then re-equilibrated with mobile pheise A for 2 min.)... 

Anticoagulant An anticoagulant is a compound that prevents the blood from clotting (coagulating). It is added in the sample tube when collecting plasma as in contrast to serum. In this respect, citrate, ethylenediaminetetraacetic acid (EDTA) and heparin are frequently used chemicals. 

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