7-Ethoxyresorufin-O-deethylase

Kannan, K., D.L. Villeneuve, A.L. Blankenship, and J.P. Giesy. 1998b. Interaction of tributyltin with 3,3, 4,4, 5-pentachlorobiphenyl-induced ethoxyresorufin O-deethylase activity in rat hepatoma cells. Jour. Toxicol. Environ. Health 55A 373-384. 

The most sensitive effect of in ovo exposures was induction of hepatic ethoxyresorufin O-deethylase (EROD) activity in 1-day-old chicks, with an ED50 dose of 0.312 pg/kg FW egg. 

Sanderson, J.T., S.W. Kennedy, and J.P. Giesy. 1998. In vitro induction of ethoxyresorufin-O-deethylase and porphyrin by halogenated aromatic hydrocarbons in avian primary hepatocytes. Environ. Toxicol. Chem. 17 2006-2018. 

Single ip injection of 30 mg PCB 118/kg BW to immatures. Fish killed 4 days later and liver analyzed Liver EROD (7-ethoxyresorufin O-deethylase) activity was 5.6 times higher than controls AHH activity was 2.7 times higher than controls 5... 

Scup, Stenotomus chrysops injected intraperitoneally with 1,5, or 10 mg/kg BW of PCBs 77, 105, 118, 128, or 138 and examined tor increases in ethoxyresorufin O-deethylase (EROD) activity, immunodetectable cytochrome P-450E (the EROD catalyst in scup) and in vitro translatable mRNA for P-450E... 

Fed diets for 4 weeks containing daily doses equivalent to 3 mg PCB 105/kg BW, 0.05 mg PCB 126/kg BW, or 4.0 mg PCB 153/kg BW. Birds were killed 3 days after final treatment and livers analyzed for porphyrins, PCB residues, and activities of ethoxyresorufin O-deethylase-(EROD), aminopyrine-/V-demethylase (APND), and aldrin epoxidase (AE)... 

Kennedy, S.W., A. Lorenzen, C.A. James, and R.J. Norstrom. 1992. Ethoxyresorufin-O-deethylase (EROD) and porphyria induction in chicken embryo hepatocyte cultures — a new bioassay of PCB, PCDD, and related chemical contamination in wildlife. Chemosphere 25 193-196. 

The measurement of the ethoxyresorufin-O-deethylase (EROD) activity is another sensitive parameter to detect the effects of paper mill industrial effluents on living organisms in the receiving waters. The EROD activity is a measure of the activity of the cytochrome P-450 enzyme system, which plays a central role in the transformation and elimination of xenobiotics. Increased EROD activity has been shown as far as 40 km from pulp mills, and EROD induction in fish caused by pulp mill effluents remains after biological treatment [60]. It is specified that EROD activity and erythrocytic nuclear abnormalities are induced by abietic and dehydroabietic acid [60]. 

M (cytoplasmic vacuolation, accentuation of zonation, increased ethoxyresorufin-O-deethylase activity) 402.9 F (cytoplasmic vacuolation, accentuation of zonation, increased ethoxyresorufin-O-deethylase activity)... 

Figure 6.2 Ethoxyresorufin-O-deethylase(EROD) induction in H4IIE rat hepatoma cells exposed to an SPMD dialysate. Four Standard SPMDs were deployed for a 28 day period in Bayou Meto, Arkansas. Doses of dialysate were normalized to gram-equivalents of triolein per mg of cellular protein. This figure was generated by Don Tillitt, USGS-CERC, Columbia, MO, USA and is reprinted with permission from the American Petroleum Institute (Huckins et al., 2002).

Whyte, J.J. Jung, R.E. Schmitt, C.J. Tillitt, D.E. 2000, Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomaiker of chemical exposure. Crit. Rev. Toxicol. 30 347-570. 

Figure 8.6 Induction of significant activity in the ethoxyresorufin-O-deethylase (EROD) screen by an extract of an SPMD sample from Lake Shkodra/Skadar. Reprinted from Rastalletal. (2004a), copyright (2004) reproduced with permission from Environmental Science and Pollution Research.

PCNs, particularly penta- and hexaCNs, induce hepatic ethoxyresorufin O-deethylase (EROD) and aryl hydrocarbon hydroxylase (AHH) activities [45,46] and oxidative stress resulting in increased lipid peroxidation, decreased hepatic vitamins A and E, and decreased catalase and superoxide... 

Abraham, K., R. Krowke, and D. Neubert. 1988. Pharmacokinetics and biological activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin. 1. Dose-dependent tissue distribution and induction of hepatic ethoxyresorufin O-deethylase in rats following a single injection. Arch. Toxicol. 62(5) 359-368. 

SANDERSON J T, AARTS J M M J G, BROUWER A, FROESE K L, DENISON M S and GIESY J P, Comparison of Ah-receptor-mediated luciferase and ethoxyresorufin O-deethylase induction in H4IIE cells implications for their use as bioanalytical tools for the detection of polyhalogenated aromatic compounds , Toxicol Appl Pharmacol 1996 137 316-25. 

Bioassays using induction of the enzymes ethoxyresorufin o-deethylase (EROD) and/or arylhydrocarbon hydroxylase (AHH) in rat hepatoma H-4-IIE cells (Zacharewski et al. 1989) and modified mouse liver cells (Schuman and Hunter 1988) have been developed and tested on water, soil, and fish samples. The... 

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