Ethanol, fluorescence Rhodamine

Fig. 5.135 Fluorescence decay curves obtained be two-photon excitation. 1) Rhodamin 6G, 10 moFl in ethanol, 2) Rhodamin 6G 10 mol/l in ethanol, 3) Fluorescein Na 10 mol/l in pH 7.4 buffer, 4) Fluorescein Na 10 mol/l in pH 7.4 buffer, 5) Laser pulse...

Feed ethanol Tracer fluorescent -Rhodamine B Fluorescence microscopy measurements Rectangular microchannel Trachsel et al. [14]... 

In most cases, the linear absorption is measured with standard spectrometers, and the fluorescence properties are obtained with commercially available spectrofluo-rometers using reference samples with well-known <1>F for calibration of the fluorescence quantum yield. In the ultraviolet and visible range, there are many well-known fluorescence quantum yield standards. Anthracene in ethanol (Cresyl Violet in methanol (commonly used reference samples for wavelengths of 350-650 nm. For wavelengths longer than 650 nm, there is a lack of fluorescence references. Recently, a photochemically stable, D-ji-D polymethine molecule has been proposed as a fluorescence standard near 800 nm [57]. This molecule, PD 2631 (chemical structure shown in Fig. 5) in ethanol, has linear absorption and fluorescence spectra of the reference PD 2631 in ethanol to... 

Logarithmic plots of the fluorescence decays for the lowest excited singlet state of H2TPP, H2OEP, and their deuterium analogues were described by single exponential forms. Fluorescence lifetimes of all compounds were obtained by deconvolution and fluorescence quantum yields were determined relative to rhodamine 640 in ethanol (32). Triplet quantum yields (0r) were determined relative to benzophenone in benzene by using the transient absorption (33). Quantum yields and lifetimes determined are summarized in "Table II". 

Fig. 11 Excited state absorption spectra of 3 (1) and 11 (2) in ACN, 17 (3) in THF and Rhodamine 6G (4) in ethanol. Normalized fluorescence of 3, 11 in ACN, and 17 in THE. Fluorescence spectrum of Rhodamine 6G in ethanol is normalized to its maximiun of amplification (curve 4). Pump energy p 350 xj at excitation wavelength Xp = 355 nm...

If one of the substances has a known fluorescence efficiency, the value of the other is then simply obtained. Convenient standard solutions are rhodamine B in ethanol with fluorescence in the yellow and efficiency 0.69, quinine bisulfate in 0.1 N sulfuric acid with fluorescence in the blue and efficiency 0.55. anthracene in ethanol with fluorescence in the violet and efficiency 0.27 in the ultraviolet region, naphthalene ( = 0.19), phenol (0 = 0.19), or benzene (0 = 0.042) can be used. With the last four compounds the solution must be deaerated by passing a current of nitrogen before measurement. To minimize the effect of errors in the spectral sensitivity curve it is desirable to use as the standard a solution... 

Figure 7.23 Absorption and excitation spectra of Rhodamine G in ethanol, (a) Corrected excitation (open circles) compared with absorption (full line), (b) Uncorrected excitation spectrum. Vertical axes, fluorescence intensity in arbitrary units...

FIGURE 13.5 (a) Visualization of a typical multilamellar ethosome containing 2% PL, 30% ethanol, and water by TEM (b) Entrapment of fluorescent probes by phopholipid vesicles as visualized by CSLM. Liposomes (a-c) or ethosomes (d-f) were prepared with one of three fluorescent probes rhodamine red (a, d), D-289 (b, e), or calceine (c, f). White represents the highest concentration of probe. (Reproduced from Touitou, E. et al., J. Control. Release, 65, 403, 2000. With permission from Elsevier.)... 

Stock solutions containing fluorescent labels DiD (l,l -dioctadecyl-3,3,3, 3 -tetramethylindocarbocyanine 4-chlorobezenesulfonate salt) (Molecular Probes, Eugene, OR, USA) or Lissamine rhodamine B labelled glycerophos-phoethanolamine (Avanti Polar Lipids, Alabaster, USA) are made in ethanol and kept at -20°C until use. These lipids are used to label the bilayer of the liposomes. 

Figure 32.1 (a) A schematic illustration of time-gated excitation-emission matrix spectroscopy, (b) A typical example of the 3D fluorescence data measured for Rhodamine 590 in ethanol... 

Spray solution Storage Substances Dissolve 100 mg rhodamine 6G in 100 ml ethanol (96%). Both reagent solutions may be stored for an extended period. Rhodamine 6G (C.l. 4S160) Method Layer Ascending, one-dunensional development in a trougli cliam- ber. HPTLC plates RP-18 without fluorescence indicator (Merck). 

Figure 5. The data processing system with actual time-resolved fluorescence measured for a 5 10 4 M rhodamine B in ethanol (I-mm pathlength) excited by a O.I-mJ, 530-nm laser pulse. The data obtained initiallyfrom the OMA are corrected by computer. In these data, the average time between points (individual OMA channels) is0.8 ps. (Reproduced with permission from Ref. 26. Copyright 1980, North-Holland Publishing Company.)...

Figure 8. Fluorescence quantum yield of 3 x 10 Af Rhodamine 6G perchlorate in ethanol versus partial pressure of dissolved oxygen. O, CjHjOH , CjHjOD.

For the cooling experiments described in the following section we used a deaerated solution of highly pure Rhodamine 6G perchlorate in monodeuterated ethanol (CjHjOD). To prevent as much reabsorption of the fluorescence as possible, a low dye concentration of 3.5 x 10" M was chosen. The absorption of this solution in the wavelength region of interest is shown in Figure 11. For the absorption measurements as well as for the quantum yield experiments, commercial spectrometer cells have been used. To prevent dye adsorption at the window surfaces the cells were rendered hydrophobic with dichlorodimethylsilane [13,21]. 

Because of the good agreement between the predicted and measured cooling effects, the fluorescence quantum yield of Rhodamine 6G in mono-deuterated ethanol has to be very close to the value 0.99 measured by the thermal leasing method. This indicates the high accuracy of this method, which does not require a luminescence standard. In fact, using the thermal... 

Figure 3.17. Fluorescence emission spectrum of Rhodamine B dissolved in ethanol. The spectrum was taken by R. A-Fiii on 6/20/9S uang an excitation wavelet ofSIO nm See Du, H., Fuh, R.A., Coikan, Li. A, Lindsey. J. S, 1998. Photochem. PhetobioL 68, 141-142. Curtos from Oregon Medical Lastf Center.

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