Fluorescence rhodamine

Gel-based ABPP enables visualization of labeling events using gel electrophoresis accompanied by in-gel fluorescence (rhodamine) or... 

ROS in skin can be detected by exogenous chromophores like dihydrorhodamine (DHR) (Figure 2.5). DHR is nonfluorescent until reaction with ROS when it becomes fluorescent rhodamine-123 (R123, = 535 nm). The reaction scheme is given in... 

FIGURE 2.6 The ROS probe dihydrorhodamine (DHR) is non-fluorescent until it reacts with ROS to form fluorescent rhodamine-123 (R1230). DHR is not a selective reactant and may react with many other ROS than those listed above. It also does not localize in nuclei nor in cell membranes and cannot identify if ROS are generated in these regions on keratinocytes. Other ROS prohes may prove to he useful to provide more data on these cellular locations. 

Luminescence is sensitive enough to observe single molecules.13 Figure 18-15 shows observed tracks of two molecules of the highly fluorescent Rhodamine 6G at 0.78-s intervals in a thin layer of silica gel. These direct observations confirm the random walk of diffusing molecules postulated by Albert Einstein in 1905. 

Introduction of an oxygen bridge into the triphenylmethane dye molecule leads to the xanthene dyes. The color is shifted from blue to red. The restricted rotation of the phenyl groups inhibits radiationless de-excitation and gives rise to very strong fluorescence. Rhodamine B (14) is used for dyeing paper [11] and as a laser dye. 

Under fluorescence (rhodamine filters), the Evan s blue fluoresces red. Healthy muscle will have positively labeled membranes, as well as blood vessels, but muscles with compromised fiber integrity will contain fibers in which the entire cross-sectioned cytoplasm is strongly fluorescent. 

Feed ethanol Tracer fluorescent -Rhodamine B Fluorescence microscopy measurements Rectangular microchannel Trachsel et al. [14]... 

More reeently, u-GalNAc liposomes were formulated by the incorporation of D-GalNAc-linked citronellol to L-a-phosphatidylcholine and rhodamine-DHPE (Fig. 10). In vitro studies on HepG2 cells, monitored by fluorescence (rhodamine), pointed out the preferentially endocytose manner of uptake. This could lead to apoptosis with the appropriate drug (Dox for example) delivered into the parenchymal cells carcinoma. 

Dai S., Sigman M.E., Burch E.L. Preparation of a photochromic glass doped with methylviologen radical cation via a sol-gel process. Chem. Mater. 1995 7 2054-2057 del Monte F., Levy D. Formation of fluorescent rhodamine B J-dimers in sol-gel glasses induced by the adsorption geometry on the silica surface. J. Phys. Chem. B 1998 102 8036-8041 Dunn B., Mackenzie J.D., Zink J.I., Stafsudd O.M. Solid-state tunable lasers based on dye-doped sol-gel materials. Proc. SPIE vol. 1328 Sol-Gel Optics 1990 1328 174-182 Dunn B., Zink J.I. Probes of pore environment and molecule-matrix interactions in sol-gel materials. Chem. Mater. 1997 9 2280-2291... 

Figure 4 Three-dimensional in vivo 2P-CLSM illustration of Fe " -mediated Fenton reaction converting nonfluorescent dihydrorhodamine 123 into fluorescent rhodamine 123 al hot spots (light gray) inside the perinuclear endoplasmatic reticulum (dark gray) of one HepG2 cell.

A new and simple method for measuring peroxides in a single living cell has been developed, and the generation of peroxides upon ultraviolet (UV) irradiation was measured in human and pig pidermal ke-ratinocytes. The method was based on the fact that the nonfluorescent dye dihydrorhodamine 123 reacts in the presence of peroxides, such as H2O2, and changes into fluorescent rhodamine 123, and hence... 

Schmued L, Kyriakidis K, Heimer L (1990) In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS. Brain Res 526 127-134... 

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