Escherichia coli genome

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For complex samples containing several different DNAs, multiplex PCR has been carried out. For instance, on-chip multiplex PCR was achieved on four DNAs representing regions in the bacteriophage A.DNA (199 and 500 bp), Escherichia coli genomic DNA (346 bp), and E. coli plasmid DNA (410 bp). After PCR, the fluorescent intercalating dye (TO-PRO) was added to the PCR reservoir, and CGE separation was performed downstream [929]. 

Uwisc http //www.genetics.wisc.edu/ Escherichia coli genome... 

Ouzounis CA, Karp PD. Global properties of the metabolic map of Escherichia coli. Genome Res. 2000 10 568-576. 

Chen S, Lesnik EA, Hall TA, Sampath R, Griffey RH, Ecker DJ, Blyn LB. A bioinformatics based approach to discover small RNA genes in the Escherichia coli genome. Biosystems 2002 65(2-3) 157-177. 

In parallel with NGS technological improvements, many algorithmic advances have been made in de novo sequence assemblers using short-read data (80). In many instances, existing computer resources in the laboratory are enough to perform de novo assemblies. The Escherichia coli genome can be assembled in as little as 15 min using a 64-bit Windows desktop computer with 32 Gb of RAM (81). 

J. S. Fetrow, A. Godzik, J. Skolnick. Functional Analysis of the Escherichia coli Genome Using the Sequence-to-Structure-... 

B. et al. (2015) Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. AppL Environ. Microbiol, 81, 2506—2514. 

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Bakthavathsalam P, Rajendran VK, Mohammed JAB. A direct detection of Escherichia coli genomic DNA using gold nanoprobes. Journal of Nanobiotechnology 2012 10 10. http //dx.doi.org/10.1186/1477-3155-10-8. 

Lukjancenko, O., Wassenaar, T.M., and Ussery, D.W. (2010). Comparison of 61 sequenced Escherichia coli genomes. 

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